Cytomegalovirus (CMV) drug resistance, Next-Generation Sequencing, plasma

Alphabetical Test listing

Cytomegalovirus (CMV) drug resistance, Next-Generation Sequencing, plasma-994

Test name:

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Alternate names:

CMV by NGS
CMV Drug resistance
CMV sequencing
Cytomegalovirus antiviral resistance
UL97
UL54
Ganciclovir
Cidofovir
Foscarnet

Useful for:

Detecting variants in the cytomegalovirus genes, UL97 and UL54, which are associated with antiviral resistance

Limitations:

This test is not useful for the initial diagnosis of CMV infection

Specimen type:

EDTA plasma

Collection container:

Lavender (EDTA), 4mL

Volume:

1.2 mL

Minimum volume:

1.0 mL

Collection instructions:

Immediately following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

Processing instructions:

Spin
Transfer plasma to a Screw cap transfer vial/tube (Mayo T914), labelled as EDTA plasma
Freeze

Transport container:

Screw cap transfer vial/tube (Mayo T914)

Transport and stability:

Frozen (preferred) – 30 days

Refrigerated – 14 days

Ambient – NO

Specimen retention time:

14 days

Performing lab:

Mayo Clinic Laboratories (CMVNG): R-NX

Days set up:

Mo - Fr

TAT:

7 – 14 days

Method:

Next-Generation Sequencing (NGS)

Reference ranges:

None detected/not predicted

Clinical information:

Next-generation sequencing testing may be indicated if a patient with known CMV infection has a rising or stabilizing viral load while on antiviral therapy. This test can assist in determining whether CMV is present that has acquired variants associated with antiviral resistance, and should only be performed on patients who have had a recent (ie, within the previous 7 days) CMV quantitative plasma viral load of 500 IU/mL or more.

CPT codes:

87910

Date created:

03/31/2021

Review date:

01/02/2024