BRG-1 by IHC

Alphabetical Test listing

BRG-1 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
BRG-1 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

FFPE tissue block

  

Tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily A, member 4) encodes BRG1, a protein of the WSI/SNF complex involved in chromatic remodeling. SMARCA4-deficient neoplasms have undifferentiated, often rhabdoid morphology and demonstrate loss of BRG1 immunohistochemical nuclear expression.

Staining pattern

Nuclear staining in normal tissues. Loss of nuclear staining is present in SMARCA4/BRG1 deficient tumors.

Small cell carcinoma of the ovary hypercalcemic type with loss of nuclear staining; lymphocytes and endothelial cells serve as positive internal controls

Applications

  • Loss of SMARCA4/BRG1 characterizes small cell carcinoma of the ovary hypercalcemic type, SMARCA4 deficient undifferentiated uterine sarcoma, and SMARCA4 deficient thoracic carcinomas/sarcomas and demonstrate rhabdoid or poorly differentiated morphology.
  • BRG1 loss has been described in a subset of melanomas, epithelioid mesotheliomas, lung adenocarcinomas, and lung squamous cell carcinomas.

 

References

  1. Conlon N, Silva A, Guerra E eta l. Loss of SMARCA4 expression is both sensitive and specific for the diagnosis of small cell carcinoma of ovary, hypercalcemic type. Am J Surg Pathol 2016;40(3):395-403.
  2. Nambirajan A, Singh V, Bhardway N et al. SMARCA4/BRG1-Deficient non-small cell lung carcinomas- A case series and review of the literature. Arch Pathol Lab Med. 2020 doi: 10.5858/arpa.2019-0633-OA

 

  
88342 - 1st stain
88341 - each additional stain
  
02/11/2021
  
02/12/2021