CD79a by IHC

Alphabetical Test listing

CD79a by IHC-12376 - Technical only, 12379 - Technical & Interpretation

  
CD79a by IHC
  
12376 - Technical only, 12379 - Technical & Interpretation
  
LAB12376
LAB12379
  
pan B cell
  

All IHC stains will include a positive control tissue

  
  • Intended for the identification of cells of B-cell lineage (reacts with 97% of B-cell neoplasms) [see Table 1]
  • Staining is consistently strong on small cell neoplasm, but large B-cell neoplasms and follicular lymphomas show weaker staining2
  • Normal plasma cells will stain intensely with CD79a
  • 50% of myelomas show CD79a positivity2
  • CD79a is found in the majority (89%) of acute leukemias of precursor B-cell type, in B-cell lines but not in T-cell lines1
  • Most reliable B-cell marker detectable in paraffin embedded specimens in precursor B-cell ALL2
  • CD79a is specifically associated with bi-phenotypic leukemias that express myeloid and B-cell antigens and have heavy chain genes in rearranged configurations. Along with CD-22, it is more useful than other B-cell antigens in detecting bi-phenotypic cases within AML
  • CD79a is an improvement on L26(CD-20) which labels dendritic reticulum cells, some T-cells and reacts with neoplastic cells in cases of AML2
  • CD20 is not expressed until the late precursors B-cell stage and at least half of precursor B ALL's are CD-20 negative3
  • Occasional Reed Sternberg cells in cases of Hodgkin's disease are CD79a positive 4 & 5


Table 1 (Ref 2)
Reactivity of Anti-CD79a (mb-1) antibody JCB117 with paraffin-embedded samples of Hematopoietic Neoplasms

B-cell neoplasms  
Small lymphocytic lymphoma/CLL 28/28
Lymphoplasmacytoid lymphoma 36/36
Mantle cell lymphoma 17/17
Follicular lymphoma 53/53
MALT lymphoma 29/29
Hairy cell leukemia 15/15
Myeloma/plasmacytoma 10/20
Large cell lymphoma 95/95
Burkitt's lymphoma 7/7
Anaplastic large cell lymphoma 13/15
Total 344/356 (97%)
   
T-cell and non-lymphoid neoplasms  
Lymphoblastic lymphoma/leukemia 0/9
Mycosis fungoides 0/10
Peripheral T-cell lymphoma 0/32
Angioimmunoblastic T-cell lymphoma 0/8
Anaplastic large cell lymphoma 0/11
Acute myeloid leukemia 0/28
Total 0/98

 

  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • The mb-1 polypeptide is part of the B-cell antigen receptor complex
  • Appears early in B-cell maturation at the pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component
  • Virtually B-cell specific

 Staining pattern

  • Cell surface membrane and cytoplasmic

References:

  1. Blood, Vol 82(3):853-857, 1993.
  2. Blood, Vol 86(4):1453-1459, 1995.
  3. AJCP, Vol 106:462-468, 1996.
  4. AJSP, Vol 19(11):1294-1299, 1995.
  5. Histopathology, 24(6):511-515, 1994.
  6. Spec sheet from DAKO.
  
88342 - 1st stain
88341 - each additional stain
  
06/16/2017
  
10/18/2018
  
10/02/2020