Trichomonas, Candida, and Bacterial vaginosis by NAA

Alphabetical Test listing

Trichomonas, Candida, and Bacterial vaginosis by NAA-14413

Trichomonas, Candida, and Bacterial vaginosis by NAA
Vaginitis probe
Vaginosis probe

Candidiasis and Bacterial Vaginosis (BV) involve overgrowth of organisms normally present in flora.

Trichomonas, Candida, and Bacterial vaginosis by nucleic acid amplification  is the preferred diagnostic test for the common causes of vaginal symptoms, including bacterial vaginosis, Candida, and Trichomonas.


  • Reliable results are dependent on adequate specimen collection, transport, storage, and processing. Because the transport system used for this assay does not permit microscopic assessment of specimen adequacy, proper specimen collection techniques are necessary.
  • Performance of the assay has not been evaluated in women less than 14 years of age
  • Collection and testing of patient-collected vaginal swab specimens with the
    assay is not intended to replace clinical examination. Vaginal infections may result from other causes or concurrent infections may occur
  • Interference with the assay was observed in the presence of the following substances:
    • Tioconazole 6.5% Ointment (3% W/V, all analytes)
    • Vaginal Moisturizing Gel (1% W/V, C spp; 5% W/V, C. glabrata 3% W/V, TV)
    • Glacial Acetic Acid (5% V/V, C spp only)
Vaginal swab
  1. Partially peel open the Multitest swab package. Remove the swab. Do not touch the soft tip or lay the swab down. If the soft tip is touched, the swab is laid down, or the swab is dropped, use a new Aptima Multitest Swab Specimen Collection Kit.
  2. Hold the swab, placing your thumb and forefinger in the middle of the swab shaft covering the score line. Do not hold the swab shaft below the score line.
  3. Carefully insert the swab into the vagina about 2 inches (5 cm) past the introitus and gently rotate the swab for 10 to 30 seconds. Make sure the swab touches the walls of the vagina so that moisture is absorbed by the swab and then withdraw the swab without touching the skin.
  4. While holding the swab in the same hand, unscrew the cap from the tube. Do not spill the contents of the tube. If the contents of the tube are spilled, use a new Aptima Multitest Swab Specimen collection kit.
  5. Immediately place the swab into the transport tube so that the score line is at the top of the tube.
  6. Carefully break the swab shaft at the score line against the side of the tube.
  7. Discard teh top portion of the swab shaft
  8. Tightly screw the cap onto the tube.

Note: A single vaginal collection container can be shared for testing of vaginal specimens submitted for Chalmydia trachomatis and Neisseria gonorrhea.


Aptima Multitest swab specimen collection kit


Ambient - 7 days

7 days
  • Improper label (unlabeled or mislabeled)
  • Time delay between time of collection and receipt of specimen
  • Improper transport container
    • Specimen not submitted in Aptima-Multitest collection vial
  • Collection vials submitted without swabs
  • Improper transport temperature
  • Interfering substances
  • Inappropriate specimen type
    • Sources other than vaginal
  • Leaking container
AHL - Microbiology: M
Routine - 2 days
Stat - 4 hours after receipt in the performing laboratory

Nucleic acid amplification test (NAAT)



Bacterial vaginosis (BV) Specificty 89.6%
Sensitivity 95.0%
Candida species group Specificty 94.9%
Sensitivity 91.7%
Trichomonas Specificty 95.1%
Sensitivity 96.5%

Positive results should be correlated in the clinical context of the patient.
Invalid result can and do occur.  Recollection is recommended if signs and symptoms warrant testing.