ANCA (anti-neutrophil cytoplasmic antibodies) occur in patients with granulomatosis with polyangitis (GPA), microscopic polyarteritis, necrotizing or crescentic glomerulonephritis, other vasculitides and inflammatory bowel disorders (primarily ulcerative colitis).
Immediatley following collection, mix sample by gently inverting 5 times
Gold serum separator (SST) tube
Refrigerated - 7 days
Immunofluorescence Assay (IFA)
If c-ANCA Screen is positive, c-ANCA titer will be performed at an additional cost.
Screening performed at 1:20 dilution.
Titers performed for positive c-ANCA and p-ANCA at the following dilutions: 1:40, 80, 160, 320, 640, 1280. ANA will be performed at 1:20 on any Atypical p-ANCA to rule out interference. Note that ANA at this titer is utilized in the laboratory for interpretation only. It is not a reportable ANA result and should not be interpreted as a positive ANA for the diagnosis of lupus or other auto-immune disease.
Positive or atypical c-ANCA and p-ANCA will reflex to Myeloperoxidase Antibodies and Proteinase 3 Antibodies, IgG at an additional charge.