High grade B-cell lymphoma panel by FISH

Alphabetical Test listing

High grade B-cell lymphoma panel by FISH-CG MYC or MYC

  
High grade B-cell lymphoma panel by FISH
  
CG MYC or MYC
  
LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES
  
CG MYC or MYC
  
BCL2/IGH
BCL6
Diffuse large B-cell lymphoma
FISH
Fluorescence in situ hybridization
High grade B-cell lymphoma
MYC
3q27
8q24
t(14;18)
14q32
18q21
  

CG MYC or MYC

If MYC results are positive for rearrangement, both BCL2/IGH and BCL6 tests will be reflexed.

  

To help differentiate diffuse large B-cell lymphoma from high grade lymphoma.

  
Tumor tissue (fresh)
  

Sterile culture medium or a sterile cup with saline

  
5 mm (3)
  

Sterile culture medium or in a sterile cup with sterile saline

  
Tumor tissue FFPE block or slides
  
Block
Slides: 2 plus one H & E stained slide
  

Fix the tissue in 10% formalin and prepare a Formalin-fixed, paraffin-embedded (FFPE) tissue block

Either the entire block, or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing

  

FFPE tissue block or

Tissue section mounted on charged, unstained slides

  

AHL Cytogenetics oncology request

  

Ambient

  
Paraffin specimens:
Slides - 6 months
Blocks will be returned post reporting

Fresh tissue – until testing is reported
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Delay in transport
  • Improper storage
  • Leaking container
  • Slides broken beyond repair
  
AHL - Cytogenetics: X
  
5 days
  

Fluorescence in situ hybridization (FISH)

  

Normal or Abnormal with interpretation

  
Paraffin specimens:

For MYC negative results, 88366 will be charged.
If MYC is positive for rearrangement, BCL2/IGH and BCL6 will be reflexed and 88366 x 3 – Qualitative Morphometric FISH codes will be charged.
If no cells are available for analysis, no analysis charges will be incurred

Fresh tissue specimens:
For MYC negative results, quantitative 88271 x 2 and 88275 x 1 codes will be charged.
If MYC is positive for rearrangement, BCL2/IGH and BCL6 will be reflexed and 88271 x 6 and 88275 x 3 codes will be charged.
If no cells are available for analysis, no analysis charges will be incurred
  
Yes
  
  
03/26/2021