p53 by IHC

Alphabetical Test listing

p53 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
p53 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
  • All IHC stains will include a positive control tissue
  

P53 over-expression is present in many different malignancies, such as colon, stomach, bladder, breast, lung, testes, melanomas, sarcomas, etc.

 

  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • P53 is a transcription factor that regulates cell growth and inhibits cells from entering S-phase
  • P53 is a DNA-binding phosphoprotein coded for by a tumor suppressor gene located on the short arm of chromosome 17
  • Mutations of p53 or functional inactivation with intact p53 genes are common in many cancers, leading to loss of normal growth regulation
  • Although p53 is present in small amounts in normal cells (not usually detected by routine immunohistochemistry), mutations resulting in a prolonged half-lives lead to accumulations that are detected by immunohistochemistry
  • Over-expression is associated with a poor prognosis in many cancers

Staining pattern

  • Nuclear staining

References

  1. Bartek J et al: Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies. Oncogene 1991; 6: 1699-703.
  
88342 - 1st stain
88341 - each additional stain
  
07/19/2017
  
10/19/2018
  
10/02/2020