Bone marrow study

Alphabetical Test listing

Bone marrow study-851

Bone marrow study
Bone Marrow aspirate

Bone marrow specimen transport kit (liquid Heparin)

  • For sites that provide their own liquid heparin to procure the 2nd aspirate


Bone Marrow Aspirate

  1. EDTA aspirate (first aspirate)
    1. minimum of 5mL of EDTA aspirate is required
    2. Extra aspirate should be placed in an EDTA tube
    3. No portion should be discarded
  2. Heparinized aspirate (second aspirate)
    1. A minimum of 10 mL of aspirate is required 
    2. Sites using liquid heparin should transfer heparinized aspirate to 2 8 mL polystyrene round bottom Falcon tubes provided in kit.
    3. Sites not using liquid heparin should transfer the aspirate to 2 sodium heparin tubes provided in the kit




  • Smears are prepared on a frosted or super-frosted slide
  • The slides must be labeled on the frosted end using a pencil or a slide/cassette labeling marker to include:
    • Patient’s full first and last name
    • Date of birth
    • Date of collection
    • Slide type (direct, unit or TP)
    • Left or right
  1. Prepare 4 Direct smears
    1. Smear made with technique used for making a blood smear

        2. Prepare 4 Unit smears

    1. Locate unit within drop of aspirate on slide
      1. Notify procurer if units are not present
    2. Soak up residual aspirate to isolate unit
    3. Lie a second slide on top of unit and gently press down to crush unit
    4. Pull slides apart in a parallel motion


  1. If syringe is not heparinized, immediately dispense into (2) Sodium Heparin tubes supplied
  2. If syringe is heparinized, transfer to (2) 8 mL polystyrene round bottom Falcon tubes for transport


  1. Prepare 4 touch prep slides
    1. Gently press core biopsy to slide 5 - 6 times at close intervals over the bottom 1/3 of the slide
    2. Roll core slightly with edge of slide every 2 - 3 touches to acquire imprints of entire core
    3. Repeat steps a and b with core biopsy from opposite side if bilateral

     2. Place core biopsy(ies) in B-plus fixative container supplied in kit

a. Cores from different sides should not be put in the same container

     3. Label the container

a. If no patient label available, label container with patient complete legal first and last name and date of birth

b. Write “core”, side of biopsy (L or R), time the core was put into B-plus fixative and initials on label



Bone marrow specimen transport kit


Bone marrow specimen transport kit (Sodium Heparin)

  • For sites that do not provide their own liquid heparin to procure the 2nd aspirate
  • Bone Marrow requests must be accompanied with both forms:
  • Complete Blood Count with Differential and Reticulocyte results are required
    • Include results with the request, or submit an EDTA whole blood (peripheral) sample with the bone marrow and testing will be performed at an additional charge


Bone marrow study samples should be transported the day of collection

     Packaging and shipping requirements

  1. Package the labeled, dried, unstained glass slides in plastic slide carriers provided in kit
  2. Place slide carriers in biohazard specimen bag and all paperwork in the outer pouch
  3. Place peripheral blood, EDTA aspirate and heparinized aspirate in 2nd biohazard specimen bag
  4. Place B-plus fixative core container(s) in a 3rd biohazard specimen bag
    1. B-plus fixative MUST be in bag separate from other specimens as B-plus fixative fumes will damage prepared slides and make them unusable
  5. Package slides and specimens in the Courier Transport bag the kit supplies were sent in
    1. If multiple bone marrows are being sent, send one (1) bone marrow per Courier Transport bag
  6. Place a courier barcode label on the Courier Transport Bag and the matching label in your log book
  7. Transport to Allina Health Laboratory with your next available courier at ambient temperatures
    1. Arrange for an unscheduled courier if necessary
  8. Do not freeze any of these samples
  • Improper label (unlabeled or mislabeled)
  • Clotted specimen
  • Improper anticoagulant or ratio
  • Hemolysis
  • Delay in transport
  • Improper storage/transport temperature
  • Slides broken beyond repair
AHL - Hematology/Special Hematology: S
Mo - Fr
1 - 2 days

Microscopic evaluation

Call for codes as they can vary
85025 - CBC/Diff (if appropriate)
85045 - Reticulocyte (if appropriate)