SALL4 by IHC

Alphabetical Test listing

SALL4 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
SALL4 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
  • All IHC stains will include a positive control tissue
  
  • SALL4 is highly sensitive in identifying seminomas, dysgerminomas, embryonal carcinomas, and yolk sac tumors (virtually 100%)
  • Of particular note, SALL4 also stains spermatocytic seminomas, along with classical seminomas
  • It is expressed in 69% of choriocarcinomas (trophoblastic cells); and 52% of teratomas
  • Sex cord-stromal tumors and trophoblastic tumors are SALL4 negative
  • SALL4 also stains most cases of teratoma (absent in mature components), and trophoblastic cells in choriocarcinomas
  • This marker is useful in the differential diagnosis of sex cord-stromal tumor versus germ cell tumor (use SALL4, a-inhibin, and calretinin), metastatic high-grade carcinoma vs. germ cell tumor (use SALL4 and OCT4), and germ cell tumor vs. lymphoma
  • Although SALL4 will stain virtually 100% intratubular germ cell neoplasia, but it will also stain non-neoplastic germ cells (use OCT4 instead)
  • SALL4 has been identified in 20% of cases or more of serous carcinoma of the ovary, urothelial high-grade carcinoma, and gastric adenocarcinoma (especially the intestinal type)
  • This marker is rarely seen in breast, colorectal, prostatic, and squamous cell carcinomas
  • SALL4 has been noted in rhabdoid tumors of the kidney and extrarenal sites and in the Wilms tumor
  • It has been occasionally seen in melanoma, desmoplastic small round cell tumor, epithelioid sarcoma, and rhabdomyosarcoma
  • Hematopoietic tumors are negative for SALL4
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • SALL4 is a zinc finger transcription factor expressed in embryonic stem cells
  • This antibody is highly sensitive in identifying germ cell tumors

Staining pattern

  • Nuclear staining

References

  1. Meittinen M et al: SALL4 Expression in Germ Cell and Non-Germ Cell Tumors; A Systematic Immunohistochemical Study of 3215 Cases. Am J Surg Pathol 2014;38:410-420).
  2. Ulbright TM et al: Best Practices Recommendations in the Application of Immunohistochemistry in Testicular Tumors; Report From the International Society of Urological Pathology Consensus Conference. Am J Surg Pathol 2014;38:e50-e59.
  
88342 - 1st stain
88341 - each additional stain
  
08/01/2017
  
10/17/2018
  
10/02/2020