Adipophilin by IHC

Alphabetical Test listing

Adipophilin by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
Adipophilin by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
ADP
ADRP
IHC
  

All IHC stains will include a positive control tissue.

  
  • This marker may be used to identify sebaceous neoplasms, including sebaceous carcinoma and sebaceoma (sensitivity is approximately 97%)
  • ADP will also stain milk fat globule membranes and lipid droplets, such as in adrenal gland (zona fasciculata), testes (Sertoli cells) and in lactating breast
  • Staining for ADP has been reported in eccrine apocrine carcinomas, xanthomatous lesions, and metastatic renal cell carcinomas (but the pattern is often granular which is considered negative for ADP

Immunostains in evaluation of possible sebaceous tumors

  ADP EMA Androgen receptor BerEp4
Sebaceous carcinoma + + + (83%) -
Squamous cell carcinoma - + - (90%) - (rare +)
Sebaceoma + + +/- (50%) -
Basal cell carcinoma - - +/- (50%) + (80%)
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

FFPE tissue block

  

Tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • Adipophilin (ADP) is a protein present on the surface of intracellular lipid droplets
  • ADP is present in sebaceous lesions (particularly well-differentiated tumors)

Staining patterns

  • Cytoplasmic membranous pattern of intracytoplasmic lipid globules
  • Granular staining is considered negative for ADP

References

  1. Ferringer T. Immunohistochemistry in dermatopathology. Arch Pathol Lab Med. 2015;139:83-105.
  2. Ansai S et al: Sebaceous carcinoma: an immunohistochemical reappraisal. Am J Dermatopathol. 2011;33(6)579-587.
  
88342 - 1st stain
88341 - each additional stain
  
04/28/2017
  
10/17/2018
  
10/02/2020