MTHFR C677T assay

Alphabetical Test listing

MTHFR C677T assay-5919

MTHFR C677T assay
Methylenetetrahydrofolate reductase
EDTA whole blood
3.0 mL

Only one (1) tube needs to be drawn for any combination of the following tests:

  • Calreticulin Exon 9 Assay
  • Factor V Leiden Mutation
  • Factor 2 Mutation
  • Hereditary Hemochromatosis
  • JAK2 V617F Mutation Detection
  • MTHFR C677T assay

Lavender (EDTA), 4mL




ACD whole blood
Sodium citrate (Na cit) whole blood

Lt blue Sodium citrate (NaCit)

Yellow ACD (A or B)



Lt blue Sodium citrate (NaCit)

Yellow ACD (A or B)


Molecular Medicare billing request

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.

  • Complete and submit the form to notify us of the need for Allina Health Laboratory to bill insurance for Molecular testing performed
  • All information requested is required in order for your request to be completed

Ambient (preferred)


  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Interfering substances
AHL - Molecular Diagnostics: D
8 days

Polymerase Chain Reaction (PCR) with RFLP analysis




Due to lack of established clinical utility, MTHFR genotyping is no longer covered by most insurances. MTHFR genotyping should not be ordered as a part of the clinical evaluation for thrombophilia or recurrent pregnancy loss; fasting homocysteine is the preferred biomarker in these cases. The use of MTHFR genotyping should be restricted to symptomatic patients who have an increased basal homocysteine. The lack of evidence to support MTHFR variant testing is detailed in a 2013 practice guideline from the American College of Medical Genetics and Genomics (ACMG). MTHFR genotyping is considered investigational for nutritional, mental health or pharmacogenic assessment. The indication for testing must be provided at the time of order. This is a genetic test and needs to be performed only once in a lifetime

This test may require preauthorization from the insurance provider. Check the payer guidelines and, if needed, obtain the pre-authorization prior to sample collection.