Only one (1) tube needs to be drawn for any combination of the following tests:
Lavender (EDTA), 4mL
Lt blue Sodium citrate (NaCit)
Yellow ACD (A or B)
Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.
Polymerase Chain Reaction (PCR) with RFLP analysis
Due to lack of established clinical utility, MTHFR genotyping is no longer covered by most insurances. MTHFR genotyping should not be ordered as a part of the clinical evaluation for thrombophilia or recurrent pregnancy loss; fasting homocysteine is the preferred biomarker in these cases. The use of MTHFR genotyping should be restricted to symptomatic patients who have an increased basal homocysteine. The lack of evidence to support MTHFR variant testing is detailed in a 2013 practice guideline from the American College of Medical Genetics and Genomics (ACMG). MTHFR genotyping is considered investigational for nutritional, mental health or pharmacogenic assessment. The indication for testing must be provided at the time of order. This is a genetic test and needs to be performed only once in a lifetime