1. Use a wooden spatula or edge of glass slide to break the vesicle and drain the fluid
2. Use a spatula or edge of new clean glass slide to scrape the base of the vesicle and gently spread the material onto another labeled clean glass slide.
3. Allow slides to air dry
4. It is preferable to submit more than one slide rather than applying successive samples of material to the same slide.
5. Label slide, How to Label a Slide for Cytological Examination

When smear has thoroughly dried, place into a slide container

Slide holder - 5 specimen

• Non-GYN Cytology request

Ambient

• Unlabeled or mislabeled specimen
• Unlabeled slide in a labeled slide container
• Leaking specimen if label has been rendered unreadable
• Slide broken beyond repair

Microscopic examination