AML panel by FISH

Alphabetical Test listing

AML panel by FISH-Order individual components; 4280H (blood) or 4280G (bone marrow)

  
AML panel by FISH
  
Order individual components; 4280H (blood) or 4280G (bone marrow)
  
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
LAB4280G - CYTOGENETIC BONE MARROW STUDIES
  
Acute Myeloid Leukemia
AML
CBFB-MYH11
Deletion 5q31
FISH
Fluorescence in situ hybridization
inv(16)
Monosomy 5
Monosomy 7
RUNX/RUNX1
t(16;16)
t(8;21)
5q-
-5
-7
  
  • CBFB
  • MLL
  • RUNX/RUNX1
  • 5Q-
  • 7Q-
  

The AML FISH panel is useful for detecting the prognostically significant cytogenetic FISH abnormalities used in Acute Myeloid Leukemia according to the NCCN guidelines.

  
Sodium heparin (Na hep) whole blood
  
  
5 mL
  
2.0 mL
  

Only one specimen tube is required to perform all ordered cytogenetics testing.

The more testing requested, the closer to the optimal volume requirements is required.

  

Dk green Sodium heparin (Na hep), no gel

  
Bone marrow
EDTA whole blood (a chromosome analysis study is not recommended for this specimen type)
  

EDTA whole blood: (not recommended)

Lavender (EDTA), 4mL

  
Bone marrow: 10 mL (minimum 5 mL)
  

Bone marrow

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

  

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel

  

EDTA whole blood:

Lavender (EDTA), 4mL

Bone marrow:

Sterile vial/tube

Dk green Sodium heparin (Na hep), no gel

  
  • AHL Cytogenetics oncology request
  

Ambient

  
Pellets - 2 years
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Leaking container
  
AHL - Cytogenetics: X
  
2 - 3 times per week
  
5 - 7 days
  

Fluorescence in situ hybridization (FISH)

  

Normal or Abnormal with interpretation.

Cytogenetic favorable prognostic indicators include: t(8;21) and inv(16) or t(16;16).

Cytogenetic intermediate prognostic indicators include: t(9;11) and abnormalities not classified as favorable or unfavorable.

Cytogenetic unfavorable prognostic indicators include: variant t(?;11) MLL rearrangements, -5 and 5q-, -7, and 17p- or TP53 deletions.

  
88271 x 8
88275 x 4
  
Yes
  
  
03/23/2021