Chromogranin A by IHC

Alphabetical Test listing

Chromogranin A by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
Chromogranin A by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  

All IHC stains will include a positive control tissue

  
  • Used in a panel for neuroendocrine tumors
  • Best used in combination with other neuroendocrine markers such as Synaptophysin, Leu-7 (and possibly NSE)
  • Neuroendocrine tumors with only a few secretory granules may show a false negative reactivity
  • 23-78% of small cell lung carcinomas stain positive with chromogranin.1 (usually focal and weak staining)
  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • Chromogranin is an acidic protein found in the secretory granules of neuroendocrine cells
  • 3 types exist; A, B, C: Type A is the most ubiquitous and is the type this antibody recognizes
  • Chromogranin stains most normal and neoplastic endocrine tissue containing secretory granules, including the following:
    o Adrenal medulla
    o Pheochromocytomas
    o Paragangliomas
    o Endocrine neoplasms of the pancreas
    o Medullary thyroid carcinoma
    o Normal C-cells of the thyroid
    o Normal endocrine cells of the GI tract
    o Carcinoids
    o Normal parathyroid
    o Normal anterior pituitary
    o Merkel cell tumors of skin
    o Small cell CA of the lung
  • The amount of staining is usually proportional to the number of secretory granules present.

Staining pattern

  • Cytoplasmic based staining; granular

References

  1. Guinee et. al.; The Spectrum of Immunohistochemical Staining of Small-Cell Lung Carcinoma in Specimens from Transbronchial and Open-Lung biopsies; American Journal of Clinical Pathology, 102:406-414, 1994
  
88342 - 1st stain
88341 - each additional stain
  
06/19/2017
  
10/18/2018
  
10/02/2020