EWSR by FISH

Alphabetical Test listing

EWSR by FISH-4280J

  
EWSR by FISH
  
4280J
  
LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES
  
CG EWSR
  
Ewing sarcoma
EWSR1
22q12
  

The t(11;22)(q24;q12) involving the EWSR1 gene located at g-band region 22q12 is the cytogenetic hallmark of Ewing sarcoma.  EWSR1 rearrangements are present in 80-90% of these tumors.

  
Tumor tissue (solid tumor or other)
  

Sterile vial/container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline.

  
5 mm (3)
  

Collect a 5 mmsample of involved tissue (solid tumor or other) and place in sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within 1 hr of procedure

  

Sterile vial/container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline.

  
FFPE Block
FFPE slides
  

Formalin vial

  
  1. Fix the specimen in 10% neutral buffered formalin for 6 - 72 hours.
  2. Prepare a formalin-fixed, paraffin-embedded (FFPE) tumor block
  3. Submit 2 unstained 5 micron cuts of involved tissue mounted on positively charged slides and 1 H & E

  or

  Sbumit the formalin-fixed, paraffin-embedded (FFPE) tumor block.      An H & E   will be cut and stained, and assessed by an HPA       pathologist.

 

  

Formalin-fixed, paraffin embedded (FFPE) block or 5 micron cuts mounted on positively charged slides

  
  • Cytogenetics oncology requisition
  

Ambient

  
Slides - 6 months
Blocks will be returned post reporting
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper storage
  • Leaking container
  • Slides broken beyond repair
  
AHL - Cytogenetics: X
  
As needed
  
5 days
  

Fluorescence in situ hybridization (FISH)

  

Positive for rearrangement:    >2.5% of 200 nuclei

Negative for rearrangement:  ≤2.5% of 200 nuclei

  
88377 – Quantitative Morphometric FISH

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred.
  
03/11/2021
  
04/06/2021