MLL by FISH

Alphabetical Test listing

MLL by FISH-4280H (blood) or 4280G (bone marrow)

  
MLL by FISH
  
4280H (blood) or 4280G (bone marrow)
  
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
LAB4280G - CYTOGENETIC BONE MARROW STUDIES
  
MLL
  
Acute Lymphoblastic Leukemia/lymphoma
B-ALL
B-cell ALL
FISH
Fluorescence in situ hybridization
Mixed Lineage Leukemia
MDS
Myelodysplastic syndrome
t(11q23;?)
  

Detecting MLL or t(11q23;?) rearrangements, which are recurring abnormalities in B-cell ALL and MDS.

This test can be ordered individually or as part of the B-ALL or MDS FISH Panels. See the panel tests for more details.

  
Sodium heparin (Na hep) whole blood
  
  
5 mL
  
2.0 mL
  
  • Only one specimen tube is required to perform all ordered cytogenetics testing.
  • The more testing requested, the closer to the optimal volume requirements is required.
  

Dk green Sodium heparin (Na hep), no gel

  
Bone marrow
EDTA whole blood (a chromosome analysis study is not recommended for this specimen type)
  

Whole blood: (not recommended)

Lavender (EDTA), 4mL

  
Bone marrow: 10 mL (minimum 5 mL)
  

Bone marrow

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

  

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel

  

Bone marrow:

Sterile vial/tube

Dk green Sodium heparin (Na hep), no gel

 

EDTA whole blood:

Lavender (EDTA), 4mL

  
  • AHL Cytogenetics oncology requisition
  

Ambient

  
Pellets - 2 years
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Leaking container
  
AHL - Cytogenetics: X
  
Mo, We, Fr
  
Routine: 5 - 7 days
STAT: Available upon request
  

Fluorescence in situ hybridization (FISH)

  

Normal or Abnormal with interpretation

MLL rearrangements are classified as unfavorable cytogenetics prognostic indicators in B-ALL according to NCCN guidelines.

A t(9;11) is classified as an intermediate cytogenetics prognostic indicator in AML.

All other variant MLL rearrangements t(?11) are classified poor/adverse cytogenetics prognostic indicator in AML.

  
88271 x 2
88275
  
Yes
  
  
03/23/2021