Herpes simplex virus (HSV) 1/2, PCR, blood or lesions

Alphabetical Test listing

Herpes simplex virus (HSV) 1/2, PCR, blood or lesions-13527

  
Herpes simplex virus (HSV) 1/2, PCR, blood or lesions
  
13527
  
LAB13527
  
HSVPCR
  
HSV Types 1 and 2, DNA PCR (Real time)
HSV 1/2 PCR
  

This test is intended for use as an aid to the diagnosis of herpes simplex virus (HSV) infections; it also differentiates HSV-1 from HSV-2. PCR testing of blood, serum or plasma samples is clinically useful ONLY in potential cases of disseminated HSV infection (neonates, immunosuppressed individuals) and not as an aid in the diagnosis of either mucosal or CNS disease.

HSV PCR is a single test that both detects the presence of HSV DNA and determines which type is present in the positive samples. There is no mechanism, therefore, for testing for HSV-1 without simultaneously testing for HSV-2.

  
Serum
  
  
1.0 mL
  
0.5 mL
  

Immediately following collection, mix sample thoroughly by gentle inversion, 5 times

  
  1. Allow sample to clot for a minimum of 30 minutes
  2. Spin within two (2) hours of sample collection

 

  

Gold serum separator (SST) tube

 

  
EDTA whole blood or plasma
ACD whole blood or plasma
Swab of lesion
  
  
Whole blood or plasma - 1.0 mL (minimum 0.5 ml)
  

Red:

  1. Allow sample to clot
  2. Spin
  3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum, within two (2) hours of sample collection

Lavender/Yellow:

  1. Spin
  2. Transfer plasma to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as the appropriate plasma type, within two (2) hours of sample collection, or submit whole blood in original collection container
  

Whole blood:

Lavender (EDTA), 4mL or Yellow ACD (A or B)

Plasma:

Transfer vial/tube with cap - 12mL (LabCorp)

                               

Swab:

Copan UTM-RT transport (purple cap)

  

Serum or plasma
Ambient (preferred) - 7 days
Refrigerated - 7 days
Frozen - 90 days

Whole blood
Ambient (preferred) - 7 days
Refrigerated - 7 days
Frozen - NO

Swab of lesion
Ambient (preferred) - 7 days
Refrigerated - 7 days
Frozen - 7 days

  

PCR testing cannot be added on to a sample that has been opened and/or used for other testing. If the sample has already been used for testing, a new specimen will need to be collected.

  
LabCorp Burlington (138651): R-LC
  
Daily
  
2 - 4 days
  

Real-time polymerase chain reaction (RT-PCR)

  

Negative

  
87529 x 2
  
Result 16130-7
  
04/05/2019
  
08/23/2021
  
08/24/2021