Oligodendroglioma by FISH

Alphabetical Test listing

Oligodendroglioma by FISH-CG OLIGO

  
Oligodendroglioma by FISH
  
CG OLIGO
  
LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES
  
CG OLIGO
  
Oligodendroglioma
1p36/LSI 1q25 and LSI 19q13/LSI 19p13
  

To aid in the differentiation of oligodendroglioma from astrocytoma brain tumors

  • Loss of both 1p and 19q, or 19q solely, is strongly correlated with gliomas of oligodendroglial morphology and is infrequent in gliomas of astrocytic origin
  • Gain of 19q is associated with gliomas of astrocytic origin
  
Malignant involved tumor tissue
  

Sterile Culture media, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

  
5 mm3
  

Specimens should be immersed in culture media, HBSS or sterile saline within 1 hour of procedure

  
Formalin-fixed, paraffin-embedded (FFPE) tissue block
4 unstained slides and 1 H&E or send formalin-fixed, paraffin embedded (FFPE) tumor block (an H&E will be cut and stained, and assessed by an HPA pathologist)
  

Formalin vial

  

Specimens should be immersed in 0% neutral buffered formalin within 1 hour of procedure

  • Specimen should be fixed for 6 -72 hours
  

FFPE or 5 micron cuts mounted on positively charged slides

  
  • Cytogenetics oncology request
  • Document time in fixation – specimen should be fixed in 10% neutral buffered formalin for 6 -72 hours
  

Ambient

  
Slides - 6 months
Blocks will be returned post reporting
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper storage
  • Leaking container
  • Slides broken beyond repair
  
AHL - Cytogenetics: X
  
As needed
  
5 days
  

Fluorescence in situ hybridization (FISH)

  

Ratio based testing of 2 probe sets: 1p/1q and 19p/19q

Deleted 1p:q ratio: <0.80
Normal 1p:q ratio:  ≥0.80

Deleted 19q:p ratio: <0.80
Normal 19p/q ratio: ≥0.80

  
88377 x 2 – Quantitative Morphometric FISH

This test includes a charge for application of the 4 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred.
  
03/11/2021
  
03/16/2021
  
04/06/2021