Plasma Cell Dyscrasia by FISH panel

Alphabetical Test listing

Plasma Cell Dyscrasia by FISH panel-4280H (blood) or 4280G (bone marrow)

Plasma Cell Dyscrasia by FISH panel
4280H (blood) or 4280G (bone marrow)
IGH rearrangement
Plasma Cell Myeloma
Myeloma FISH panel
Trisomy 5
Trisomy 9
Trisomy 15
14q32.3 rearrangement
TP53 deletion
17p13.1 deletion
1q21.3 gain
1p32.3 loss
  • P18
  • 5/9/15
  • TP53
  • IGH with reflex to:
    •   CCND1/IGH
    •   IGH/MAF
    •   IGH/MAFB
    •   CCND3/IGH
    •   FGFR3/IGH

Detecting abnormalities of plasma cell dyscrasias, including Plasma Cell Myeloma and to provide myeloma cytogenetic risk stratification information for mSMART 3.0.

Sodium heparin (Na hep) whole blood
5 mL
2 mL

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting


Dk green Sodium heparin (Na hep), no gel

Bone marrow
EDTA whole blood (a chromosome analysis study is not recommended for this specimen type

EDTA whole blood:

Lavender (EDTA), 4mL


Bone marrow - 10 mL (minimum 5 mL)

Bone marrow

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube


- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel


  • Only one specimen tube is required to perform all ordered cytogenetics testing.
  • The more testing requested, the closer to the optimal volume requirements is required.



EDTA whole blood:

Lavender (EDTA), 4mL

Bone marrow:

Sterile vial/tube

Dk green Sodium heparin (Na hep), no gel


  • AHL Cytogenetics oncology request


Pellets - 2 years
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Leaking container
AHL - Cytogenetics: X
As needed
5 - 7 days

Fluorescence in situ hybridization (FISH)


Normal or Abnormal with interpretation

Standard Risk mSMART 3.0 abnormalities include:

  • a trisomy result of 5, 9, or 15 (or a combination of these)
  • a t(6;14) (CCND3/IGH) rearrangement
  • a t(11;14) (CCND1/IGH) rearrangement.

High Risk mSMART abnormalities include:

  • a t(4;14) (FGFR3/IGH) rearrangement
  • a t(14;16) (IGH/MAF) rearrangement
  • a t(14;20) (IGH/MAFB) rearrangement

Of note, one high risk abnormality may be ameliorated by a trisomy finding.

Double Hit Myeloma: Any 2 high-risk abnormalities present

Triple Hit Myeloma: 3 or more high risk abnormalities present

Normal or abnormal with no reflex IGH testing:
88271 x 9
88275 x 4

Abnormal with a CCND1/IGH reflex
88271 x 11
88275 x 5

Abnormal with a full IGH reflex:
88271 x 19
88275 x 9