CD5 by IHC

Alphabetical Test listing

CD5 by IHC-12376 - Technical only, 12379 - Technical & Interpretation

Test name:

CD5 by IHC

Test number:

12376 - Technical only, 12379 - Technical & Interpretation

Excellian order number:

LAB12376
LAB12379

Tests included:

All IHC stains will include a positive control tissue

Useful for:

CD5 is useful in a panel of immunostains (on frozen and/or paraffin sections) to differentiate between various types of low grade B-cell lymphoma (CLL/WDLL and IDL/mantle cell lymphomas are positive)
CD5 should always be interpreted in conjunction with other T-cell markers and considered as positive (on B-cells) if the staining is present in areas otherwise negative for other T-cell antigens
The absence of CD5 reactivity in the above mentioned small cell lymphocytic malignancies significantly correlated with extra-nodal presentation
CD5 may be helpful in the differential of mediastinal tumors; thymic carcinomas are positive, squamous cell carcinomas are negative

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin embedded tissue block

Transport container:

Formalin-fixed, paraffin embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

CD5 is a 67 kD cell membrane glycoprotein
CD5 reacts with normal T cells, a small subset of normal B lymphocytes and neoplastic B lymphocytes in certain low grade B cell lymphomas
CD5 can also react with mesotheliomas, but shows intracytoplasmic staining rather than cell membrane based staining

Staining pattern

Cell membrane (intracytoplasmic staining seen in mesotheliomas)

References

Arber DA et al: CD5: a review. Appl Immunohistochemistry. 1995; 3:1-22.
Tjateyama H et al: Immunoreactivity of a new CD5 antibody with normal epithelium and malignant tumors including thymic carcinoma. Am J Clin Pathol 1999; 111:235-240.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

05/15/2017

Revised date:

10/18/2018

Review date:

01/12/2024