T Cell leukemia/lymphoma

Alphabetical Test listing

T Cell leukemia/lymphoma-12420

  
T Cell leukemia/lymphoma
  
12420
  
LAB12420
  
Full T panel
  
Chronic T cell leukemia
Flow
Flow Cytometry
Immunophenotyping
Lymphocyte
NK cell
T-ALL
T cell leukemia
T-LGL
  
Tubes:
T1
T2

Markers:
CD2
CD3
CD4
CD5
CD7
CD8
CD10
CD16
CD19
CD45
CD56
CD57
HLA-DR
TCR alpha beta
TCR gamma delta
TRBC1
*May vary based upon immunophenotype of cells in the specimen
  

Determine abnormal T cell lymphoproliferative phenotype

  
EDTA whole blood
  
  
4 mL
  
1.0 mL
  

Lavender (EDTA), 4mL

  
Body fluid
Bone marrow
Cerebrospinal fluid (CSF)
Tissue
  
Varies; see Alternate collection instructions
  

Body fluid

  • Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
    • Do not add anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Include representative slides (will be returned)

Bone marrow

  • Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
    • Specimens collected in EDTA are acceptable, but not preferred
  • Transfer to an anticoagulant free sterile tube
  • If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable
  • Submit representative slides (will be returned)

Cerebrospinal fluid (CSF)

  • Collect 5 mL (minimum 1 mL) in a sterile vial/container.
    • Do NOT add any anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Send representative slides (will be returned)

Tissue

  • Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
  • Send representative slides (will be returned)
  

Body fluid

Falcon vial/tube - 15mL

Bone marrow

Bone marrow transport vial/tube

Cerebrospinal fluid (CSF)

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

  

Manual request clients: Complete the "Brief clinical information" and "What do you want answered by Flow?" areas on the request form. Include previous Allina case number, if applicable.

  

EDTA whole blood

Ambient

Body fluid

Refrigerated

Bone marrow

Ambient

Cerebrospinal fluid (CSF)

Refrigerated

Tissue

Refrigerated

  
14 days
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
    • Tissues submitted in saline or B5 fixative are not acceptable and will be rejected
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Inappropriate timing of collection
  • Interfering substances
  • Leaking container
  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow cytometry

  

Tubes and Markers:

Tubes:

T1, T2, T3*

T3 tube reflexed for an additional charge, only if immature or lymphoblastic features are detected in the T1 and/or T2 tubes

Markers:

CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD16, CD19, CD45, CD56, CD57, HLA-DR, TCRαβ, TCRγδ, TRBC1

* If T3 tube is reflexed, additional markers include: CD1a, CD3(cytoplasmic), CD34, TdT(nuclear))

  
88184
88185 x 15*

*May vary based upon the immunophenotype of cells in the specimen
  
08/05/2021
  
11/23/2021
  
11/23/2021