A single stool specimen cannot be used to rule out bacteria as a cause of diarrhea. It is recommended that two or three stool specimens, collected on separate days, be submitted to increase the probability of isolating a bacterial pathogen.
Specimen should be collected in sterile bedpan, not contaminated with urine, residual soap, or disinfectants. Those portions of stool which contain pus, blood, or mucus should be submitted for analysis.
Check the expiration date of transport; do NOT use expired devices. Fecal specimens for different tests often need different transport containers and different transport conditions (eg, frozen, raw stool). Specimens should be portioned out to separate devices of each type for each test requested before sending to the laboratory. Only a thumbnail-size portion of stool, about 1 g or 1 mL, should be added to the vial. Overfilling the vial will reduce recovery of stool pathogens.
Add stool to the ParaPak C&S container until the liquid reaches the indicated fill line.
Pass swab beyond anal sphincter, carefully rotate, and withdraw. Swabbing of lesions of rectal wall or sigmoid colon during proctoscopy or sigmoidoscopy is preferred.
Duodenal or sigmoid aspirate:
Specimen should be collected by a physician trained in this procedure. Stool specimen can be divided for other types of cultures by laboratory. Miscellaneous tests and ova and parasites tests should be split into appropriate containers and transport devices prior to shipping to the laboratory.
Ambient - 72 hours
An interpretive report will be provided