Pyruvic acid, whole blood

Alphabetical Test listing

Pyruvic acid, whole blood-994

  
Pyruvic acid, whole blood
  
994
  
LAB994
  
MSO
  
Pyruvate
  

Increased pyruvic acid levels have been associated with diabetes mellitus, vitamin deficiencies, uremia, congestive heart failure, liver diseases, muscular dystrophy, thiamine deficiency, and neoplastic conditions.

Pyruvic acid is useful in assessing oxygen deprivation and provides an index of the severity of circulatory failure.

  
Potassium oxalate/sodium fluoride whole blood
  
  
0.5 mL
  
0.2 mL
Submitting the minimum volume does not allow for repeat testing
  

Draw blood into chilled gray-top tube.

  
  1. Immediately after blood is drawn, add 2 mL of whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). Perchloric acid tube
    1. Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained.
  2. Shake mixture vigorously for about 30 seconds.
  3. Refrigerate for five minutes to ensure complete protein precipitation.
  4. Centrifuge 5 to 10 minutes at approximately 1500xg.
  5. Immediately transfer the CLEAR SUPERNATANT to a Transfer vial/tube with cap - 12mL (LabCorp).
    1. A second centrifugation of the supernatant may be necessary to obtain a clear protein-free solution.
  6. Label the transport tube as "SUPERNATANT" and send to the laboratory.
  

Transfer vial/tube with cap - 12mL (LabCorp)

  

Ambient (preferred) - 5 days

Refrigerated - 7 days

Frozen - 7 days

  
  • Whole blood received
  • Grey-top tube not prepared with perchloric acid
  
LabCorp Burlington (004788): R-NX
  
Tu, Th
  
1 - 5 days
  

Pyruvate - lactate; spectrophotometry

  

0.3 - 0.7 mg/dL

  
84210
  
Result 2905-8
  
05/23/2019
  
12/29/2019