Pepsinogen I
Pepsinogen I-994
Pepsinogen I is produced primarily in the Oxyntic gland mucosa of the stomach. It is secreted mainly into the gastric lumen and into circulation. Pepsinogen I has little or no biological activity but in acid is converted to the active enzyme Pepsin which exhibits proteolytic actions. Pepsinogen I is cleared by the kidney and secreted into the urine. Patients with pernicious anemia and gastrectomized patients have low to non-detectable levels of Pepsinogen I. Pepsinogen I levels are slightly elevated in gastric ulcer, higher in gastroduodenal ulcer and significantly elevated in duodenal ulcer. Patients with Zollinger-Ellison's syndrome exhibit greatly elevated levels. Pepsinogen I has been shown to correlate with presence of genetic duodenal ulcer, and has been used as a subclinical marker of increased risk for stomach cancer.
- Patient should be fasting 10-12 hours prior to collection of specimen
- Antacids or other medications affecting stomach acidity or gastrointestinal motility should be discontinued, if possible, for at least 48 hours prior to collection.
Immediately following collection, mix sample by gently inverting 5 times
- Allow sample to clot for a minimum of 30 minutes
- Spin within 45 minutes of specimen collection
- Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum
- Freeze immediately
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting
- Spin to separate plasma from the cells within 45 minutes of specimen collection
- Transfer plasma to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as EDTA plasma
- Freeze immediately
Frozen (strict) - 365 days
Refrigerated - NO
Ambient - NO
Direct Radioimmunoassay (Direct RIA)
An interpretive report will be provided