Helicobacter pylori by IHC

Alphabetical Test listing

Helicobacter pylori by IHC-12376 - Technical only, 12379 - Technical & interpretation

Helicobacter pylori by IHC
12376 - Technical only, 12379 - Technical & interpretation
  • All IHC stains will include a positive control tissue
  • This antibody identifies H. pylori in tissue sections
  • H. pylori has been found to be associated with chronic active gastritis and in the development of peptic ulcer disease
  • HLO is useful in identifying scant numbers of HLO
  • HLO detection has been shown to improve the rate of identification of the organisms after therapy, even when histologic examination and cultures were falsely negative

Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • HLO reacts with a protein found in HLO organisms
  • The HLO antibody may react with only portions of the organism, and may label non-viable organisms
  • This antibody may also react with other bacteria, and therefore positive staining must be evaluated in light of the organism morphology

Staining pattern

  • Cell membrane


  1. El-Simaity HMT et al: Inter-observer variation in the histopathological assessment of HLO gastritis. Hum Pathol 1996; 27:35-41.
  2. Barbosa AJ et al: Immunocytochemical identification of HLO in gastritis and correlation with culture. Arch Pathol Lab Med 1988; 11:288-291.
  3. Toulaymant M et al: Endoscopic biopsy pathology of HLO gastritis. Arch Pathol Lab Med 1999; 123:778-781.
  4. Angelucci ML et al: Anti-HLO specific antibody immunohistochemistry improves the diagnostic accuracy of HLO in biopsy specimens from patients treated with triple therapy. Am J Gastroenterol 1998; 93:223-226.
88342 - 1st stain
88341 - each additional stain