OCT-2 by IHC

Alphabetical Test listing

OCT-2 by IHC-12376 - Technical only, 12379 - Technical & Interpretation

  
OCT-2 by IHC
  
12376 - Technical only, 12379 - Technical & Interpretation
  
LAB12376
LAB12379
  
  • All IHC stains will include a positive control tissue
  
  • This antibody is typically used in conjunction with Pax 5
  • Oct-2 is used to confirm B-cell lineage in CD20 negative B-cell lymphomas (Pax-5 positive and Oct-2 positive)
  • This antibody can be used to confirm plasma cell lineage, including myeloma and plasmablastic lymphomas (Pax-5 negative but Oct-2 positive)
  • Oct-2 can also be useful in phenotyping Hodgkin lymphoma (Pax-5 positive but Oct-2 negative)
  • Expression of this antibody has also been reported in AML (30% in one study, in cases with myelomonocytic differentiation)
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • Oct-2 is a B-cell lymphocyte-specific transcription co-activator (associated with BOB.1), which has a role in germinal center formation and immunoglobulin productions (i.e. involved in the earliest stage of B-cell development)
  • Oct-2 stains immunoglobulin secreting cells (B-cells and plasma cells)
  • This antibody also recognizes neuronal cells


Staining pattern

  • Nuclear staining

References

  1. Ponzoni et al: New transcription factors in diagnostic hematopathology. Adv Anat Pathol. 2007 Jan;14(1):25-35. Review.
  2. Gibson SE et al: Expression of the B cell-associated transcription factors PAX5, OCT-2, and BOB.1 in acute myeloid leukemia: associations with B-cell antigen expression and myelomonocytic maturation. Am J Clin Pathol. 2006 Dec;126(6):916-24.
  3. Cue et al: Lineage determination of CD20- B-Cell neoplasms: an immunohistochemical study. Am J Clin Pathol. 2006 Oct;126(4):534-44.
  4. McCune RC et al: Expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in Hodgkin's and non-Hodgkin's lymphomas: a comparative study using high throughput tissue microarrays. Mod Pathol. 2006 Jul;19(7):1010-8. Epub 2006 Apr 28.
  5. Brown P et al: The B-cell transcription factors BSAP, Oct-2, and BOB.1 and the pan-B-cell markers CD20, CD22, and CD79a are useful in the differential diagnosis of classic Hodgkin lymphoma. Am J Clin Pathol. 2003 Nov;120(5):767-77.
  
88342 - 1st stain
88341 - each additional stain
  
07/17/2017
  
10/19/2018
  
10/02/2020