Reptilase time

Alphabetical Test listing

Reptilase time-994

  
Reptilase time
  
994
  
LAB994
  
MSO
  

Diagnosis of fibrinogen deficiency, both congenital and acquired, as well as screen for dysfibrinogenemia. Normal values for reptilase time when thrombin time is prolonged suggests the presence of heparin or a direct thrombin inhibitor.

  
Sodium citrate (Na cit) plasma
  

Lt blue Sodium citrate (NaCit)

  
2.0 mL
  
0.5 mL
  
  1. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate filled to completion to ensure a proper blood to anticoagulant ratio.
  2. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anti- coagulant with the blood.
  3. A discard tube is not required prior to collection of coagulation samples except when using a winged blood collection device (i.e. 'butterfly'), in which case a discard tube should be used.[
  4. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes.
  5. Any tube containing an alternate anticoagulant should be collected after the blue- top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes.
  
  1. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells.
  2. Deliver to a plastic transport tube, cap, and recentifuge for 10 minutes.
  3. Use a second plastic pipette to remove plasma, staying clear of the platelets at the bottom of the tube.
  4. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap.
  5. Freeze immediately and maintain frozen until tested.
  

Frozen (strict)

  
  • Severe hemolysis 
  • Clotted specimen
  • Specimen diluted with I.V. fluids
  • Samples thawed in transit
  
LabCorp Burlington (117180): R-NX
  
Daily
  
1 - 3 days
  

The reptilase time measures the rate of fibrin clot formation after the addition of reptilase, a proteolytic enzyme derived from the venom of Bothrops atrox, to citrated plasma.

  

0.0 - 21.9 sec

  
85635
  
No
  
Result 6683-7
  
05/23/2019
  
05/23/2019