Hemoglobin (Hb) solubility

Alphabetical Test listing

Hemoglobin (Hb) solubility-13333

  
Hemoglobin (Hb) solubility
  
13333
  
LAB13333
  
HSS
  
Hb S
Hemoglobin S
Hgb solubility
Sickle cell preparation
Sickle cell solubility test
Sickle cell test
Sickle prep
Sickledex
  
  • Qualitative determination of presence of hemoglobin S
  • Detect sickling hemoglobins
  • Evaluate hemolytic anemia, undiagnosed hereditary anemia with morphologic (sickle-like) abnormalities on peripheral blood smear
  
EDTA whole blood
  
  
1.0 mL
  
0.1 mL
  

Do not spin 

  

Lavender (EDTA), 4mL

  
Sodium citrate (Na cit) whole blood
ACD whole blood
Heparin whole blood
  

Lt blue Sodium citrate (NaCit) - 2.7mL

Yellow ACD (A or B)

 

Dk green heparin (Li or Na), no gel

 

  

Lt blue Sodium citrate (NaCit)

Yellow ACD (A or B)

Dk green heparin (Li or Na), no gel

 

  
Ambient (preferred) - 14 days

Refrigerated - 14 days

Frozen - 14 days
 
Freeze/thaw cycles - stable x 3
  
  • Specimen other than whole blood received
  • Clotted specimen
  
LabCorp Burlington (005223): R-LC
  
Mo - Fr
  
1 - 3 days
  

Sodium hydrosulfite reduction

  

Negative

  
Distinction between Hb S beta-thalassemia and sickle cell anemia is not always possible on clinical, hematologic, or electrophoretic grounds. Thalassemia heterozygotes have hypochromia and microcytosis, but overlap values exist. Differentiation can best be made by family or molecular pathology methods. Regional prevalence in the midwest area of Hb S beta-thalassemia is estimated to be 1:23,000 of the black population. It is recommended that positive sickle cell patients be further evaluated with Hb fractionation (HPLC), Hb F studies, and family studies. Complete characterization may require sophisticated laboratory studies with DNA amplification.
  
85660
  
04/05/2019
  
11/11/2019
  
02/23/2021