5q31 Deletion/Monosomy 5 by FISH
5q31 Deletion/Monosomy 5 by FISH-4280G (bone marrow) or 4280H (blood)
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
AML
Deletion 5q31
MDS
Monosomy 5
Myelodysplastic Syndrome
5q-
FISH
Fluorescence In Situ Hybridization
MPN
Myeloproliferative Neoplasm
Deletion 5q is the most common structural rearrangement in myelodysplastic (MDS) syndromes and myeloproliferative neoplasms particularly idiopathic myelofibrosis (IMF). It can also be seen in and acute myeloid leukemia (AML) usually within a complex karyotype as well as in therapy-related MDS and AML, following previous exposure to alkylating agents. Monosomy 5 (-5) is a recurring abnormality in AML.
This test can be ordered individually or as part of the AML or MDS FISH Panels. See these panel tests for more details.
Bone marrow aspirate:
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
- Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
- Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting
Transfer aspirate from syringe to transport container.
Submit entire specimen unspun.
Whole blood
Touch Prep Slides
Bone Marrow Core:
Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline
or
Whole blood:
Dk green Sodium heparin (Na hep), no gel
Whole blood: 5 mL
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting.
Submit entire specimen unspun.
AHL Cytogenetics Oncology Request
Ambient
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Delay in transport
- Improper storage
- Leaking container
- Slides broken beyond repair
Fluorescence in situ hybridization (FISH)
Normal or Abnormal with interpretation.