Alphabetical Test listing

TRAcP by IHC-12376 - Technical only, 12379 - Technical & interpretation

12376 - Technical only, 12379 - Technical & interpretation
Tartrate resistant acid phosphatase
  • All IHC stains will include a positive control tissue
  • Anti-TRAcP will react with ~93% of Hairy Cell Leukemias with the percentage of TRAP-positive cells ranging from 5% to 10% (minority of cases) to >90%. Reactivity has been shown in marginal zone lymphoma of the spleen (2 of 3 cases). Other lymphoid neoplasms are negative for anti-TRAcP reactivity (see Table 3). Anti-TRAcP reactivity is not diagnostic for Hairy Cell Leukemia and should be used in conjunction with other antibodies in a panel: DBA.44, CD20, MB2.
  • Reactivity may be seen in Kupffer cells of the liver, in pulmonary alveolar macrophages, macrophages and epithelioid cells in or around granulomas and macrophages surrounding neoplasms
  • Weak TRAP positivity can be seen in occasional non-neoplastic small lymphocytes and in a subset of granulocytes or monocytes in normal bone marrow. Bone marrow osteoclasts, and Langerhans' cells in lymph nodes involved by dermatopathic lymphadenopathy may be reactive. Mast cells in some cases of systemic mastocytosis and macrophages of Gaucher's disease have shown positivity to Anti-TRAcP
  • In cases of chronic myelogenous leukemia or chronic myelomonocytic leukemia, a small number of granulocytes or monocytes have shown weak positivity. These, however, were morphologically distinct and would not be confused with hairy cells on the basis of their unique cytologic characteristics

Submit a formalin-fixed, paraffin embedded tissue block


Formalin-fixed, paraffin embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • Reacts with the 16kD subunit of human tartrate resistant acid phosphatase (TRAcP)

 Staining pattern

  • Cytoplasmic (strong granular staining)


  1. Hoyer, et. al.: immunohistochemical Demonstration of Acid Phosphatase Isoenzyme 5 (Tartrate-Resistant) in Paraffin Sections of Hairy Cell Leukemia and Other Hematologic Disorders; American Journal of Clinical Pathology, 1997; 108:308-315.
  2. Yaziji, et. al.: Immunohistochemical Detection of Tartrate-Resistant Acid Phosphatase in Non-Hematopoietic Human Tissues; American Journal of Clinical Pathology, 1995; 104:397-402.
  3. Janckila, et. al.: Hairy Cell Identification of Immunohistochemistry of Tartrate-Resistant Acid Phosphatase. Blood, Vol. 85, No. 10 (May 15), 1995: pp 2839-2844.
88342 - 1st stain
88341 - each additional stain