Cytokeratin high molecular weight by IHC

Alphabetical Test listing

Cytokeratin high molecular weight by IHC-12376 - Technical only, 12379 - Technical & interpretation

Cytokeratin high molecular weight by IHC
12376 - Technical only, 12379 - Technical & interpretation

All IHC stains will include a positive control tissue

  • In normal prostate, CK HMW stains the basal layer of prostatic glands in a membranous/cytoplasmic pattern
  • This antibody will not stain malignant prostate glands (due to the loss of the basal layer)

Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • CK HMW stains epithelium that contains high-molecular weight cytokeratin

Staining pattern

  • Cytoplasmic


  1. Brimo F, Epstein JI: Immunohistochemical Pitfalls in Prostate Pathology. Hum Pathol 2012;43(3):313-324.
  2. Hameed O, Humphrey PA: Immunohistochemistry in the Diagnosis of Minimal Prostate Cancer. Current Diagn Pathol 2006;12:279-291.
  3. Martens MB, Keller JH: Routine Immunohistochemical Staining for High-Molecular Weight Cytokeratin 34-beta and alpha-Methylacyl CoA Racemase (P504S) in Postirradiation Prostate Biopsies. Mod Pathol 2006;19:287-290.
  4. Varma M, Morgan M, Amin MB, et al: High Molecular Weight Cytokeratin Antibody (Clone 34beta12): a Sensitive Marker for Differentiation of High-Grade Invasive Urothelial Carcinoma from Prostate Cancer. Histopathol 2003;42:167-172.
88342 - 1st stain
88341 - each additional stain