Alphabetical Test listing

HBME-1 by IHC-12376 - Technical only, 12379 - Technical & interpretation

12376 - Technical only, 12379 - Technical & interpretation
  • All IHC stains will include a positive control tissue
  • Useful in identifying mesothelial cells (benign and malignant), although this marker cross-reacts with many tumors of other sites (such as from ovarian, lung, and GI tract)
  • This marker is most useful in identifying papillary thyroid cancer; diffuse and intense membranous staining favors a papillary cancer
  • Useful in battery with CK19 in discriminating papillary thyroid cancer from benign entities
  • In a recent report 2, HMBE1 staining was observed in 87 % of papillary thyroid cancers, including 43 of 49 (88%) classic papillary thyroid cancers and 25 of 29 (86%) follicular variants of papillary thyroid cancer
  • CK19 staining has been reported in 96% of papillary thyroid cancers, including 100% of classic papillary thyroid cancer and in 26 of 29 cases (90%) of follicular variants of papillary thyroid cancer 2
  • The co-expression of HBME1 and CK19 has been reported as 100% specific and 83% sensitive 2
  • HMBE1 staining may rarely be seen in follicular adenomas (2 of 49 cases; 4%); this staining pattern is usually focal, rarely diffuse and strong. CK19 has been seen in 14% (7 of 29) of follicular adenomas
  • In one study, 73% of lung adenocarcinomas are positive for this marker

Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • HBME-1 reacts with an unknown antigen in the microvilli of mesothelial cells
  • This antibody also reacts with a variety of benign and malignant epithelial cells
  • HBME-1 will stain the majority of papillary thyroid cancers (including follicular variants); a negative result helps rule against a papillary cancer
  • Non-specific staining for this marker can be seen in benign thyroid lesions and occasionally in follicular carcinomas

Staining pattern

  • Membranous staining is most useful (cytoplasmic staining can also be seen)


  1. Miettinen M et al: HBME-1, a monoclonal antibody useful in the differential diagnosis of mesothelioma, adenocarcinoma and soft-tissue and bone tumors. Appl Immunohistochem 1995; 3(2):115.
  2. Scognamiglio T et al: Diagnostic usefulness of HBME1, Galactin-3, CK19, and CITED1 and evaluation of their expression in encapsulated lesions with questionable features of papillary thyroid carcinoma. Am J Clinc Pathol 2006; 126:700-708.
  3. Nasr M et al: Immunohistochemical markers in diagnosis of papillary thyroid carcinoma: utility of HBME1 combined 
88342 - 1st stain
88341 - each additional stain