Rearrangement of the FUS gene (located at G-band region 16p11) is implicated in several chromosomal translocations associated with both solid tumors and leukemias. A t(7;16)(q33;p11) involving the FUS gene is a characteristic feature of low grade fibromyxoid sarcoma and a t(12;16)(q13;p11) resulting in the fusion of the DDIT3 and FUS genes is present in more than 90% of myxoid and round cell liposarcomas.
Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline.
Specimens should be immersed in sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one (1) hour of procedure
Either the entire block, or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing
FFPE tissue block or
Tissue section mounted on charged, unstained slides
Fluorescence in situ hybridization (FISH)
Positive for rearrangement: >2.5% of 200 nuclei
Negative for rearrangement: ≤2.5% of 200 nuclei