FUS by FISH

Alphabetical Test listing

FUS by FISH-4280J

Test name:

FUS by FISH

Test number:

4280J

Excellian order number:

LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES

Abbreviation:

CG FUS

Alternate names:

FUS
LGFMS
Low-grade fibromyxoid sarcoma
16p11

Useful for:

Rearrangement of the FUS gene (located at G-band region 16p11) is implicated in several chromosomal translocations associated with both solid tumors and leukemias. A t(7;16)(q33;p11) involving the FUS gene is a characteristic feature of low grade fibromyxoid sarcoma and a t(12;16)(q13;p11) resulting in the fusion of the DDIT3 and FUS genes is present in more than 90% of myxoid and round cell liposarcomas.

Specimen type:

Tumor tissue

Collection container:

Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline.

Volume:

5 mm (3)

Collection instructions:

Specimens should be immersed in sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one (1) hour of procedure

Alternate specimen type:

Tumor tissue FFPE block
Tumor tissue FFPE or slides

Alternate specimen volume:

Block
Slides: 2 plus one H & E stained slide

Alternate processing instructions:

Fix the tissue in 10% formalin for 6 - 72 hours.
Prepare a Formalin-fixed, paraffin-embedded (FFPE) tissue block

Either the entire block, or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing

Alternate transport container:

FFPE tissue block or

Tissue section mounted on charged, unstained slides

Required forms/information:

Cytogenetics oncology requisition

Transport and stability:

Ambient

Specimen retention time:

Slides - 6 months
Blocks will be returned post reporting

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Improper storage
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Cytogenetics: X

Days set up:

As needed

TAT:

5 days

Method:

Fluorescence in situ hybridization (FISH)

Reference ranges:

Positive for rearrangement: >2.5% of 200 nuclei

Negative for rearrangement: ≤2.5% of 200 nuclei

CPT codes:

88377 – Quantitative Morphometric FISH

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred.

Date created:

03/11/2021

Review date:

04/06/2021