Antihistone antibodies

Alphabetical Test listing

Antihistone antibodies-13350

  
Antihistone antibodies
  
13350
  
LAB13350
  
HST
  
DIL
Drug induced Lupus antibodies
H2a/H2b and H3 and H4 antibodies
  

Semiquantitative result of IgG class antibodies

  

Histones are lysine-rich and arginine-rich basic proteins of eukaryotic cells which are predominantly found as complexes with DNA. Antibodies to histones are detected in approximately 30% to 60% of patients with systemic lupus erythematosus (SLE), but their presence in about 95% of patients with drug-induced lupus is more important diagnostically.

  
Serum
  
  
1.0 mL
  
0.5 mL
Note: This volume does not allow for repeat testing
  

Spin

  

Gold serum separator (SST) tube

  
  

Ambient (preferred) - 14 days
Refrigerated - 14 days
Frozen - 14 days

  
  • Hemolysis
  • Icterus
  • Lipemia
  • Bacterial contamination
  
LabCorp Burlington (012518): R-LC
  
Tu, Th
  
2 - 6 days
  

Enzyme-linked immunosorbent assay (ELISA)

  

Negative:             <1.0 units
Weak positive:        1.0−1.5 units
Moderate positive:   1.6−2.5 units
Strong positive:     >2.5 units

  

Drug-induced lupus (primarily caused by procainamide and hydralazine) is clinically similar to SLE, except that the former patients do not have kidney or central nervous system involvement and they do not demonstrate the multiple types of antinuclear antibodies (ANAs) often found in SLE patients. In addition, drug-induced antihistone antibodies react strongly withH2a/H2b fragments (in procainamide-induced SLE) as well as H3 and H4 (in hydralazine-induced SLE). In patients with drug-induced lupus, antihistone antibodies are usually the only ANAs, although antibodies to denaturated DNA have been detected in a small percentage of patients. Antihistone antibodies also occur in approximately 20% of patients with rheumatoid arthritis.

  
86235
  
03/27/2019
  
03/29/2021
  
02/16/2021