Residual acute myeloid leukemia

Alphabetical Test listing

Residual acute myeloid leukemia-12420

  
Residual acute myeloid leukemia
  
12420
  
LAB12420
  
RAML
  
Acute Leukemia
Blast
Flow
Flow Cytometry
Immunophenotyping
Lymphoblast
MDS
Myeloblast
Precursor
Residual AML
  
Tubes:
A3 (8C)
A4 (8C)
A5 (8C)

Markers:
CD11b
CD13
CD14
CD15
CD33
CD34
CD38
CD45
CD56
CD64
CD65
CD117
CD123
CD371
HLA-DR
  

Monitor residual AML blasts based on pre-treatment phenotype

  
EDTA whole blood
  
  
4 mL
  
1.0 mL
  

Lavender (EDTA), 4mL

  
Body fluidBone marrowSpinal fluid (CSF)Tissue
  
Varies; see Alternate collection instructions
  

Body fluid

  • Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
    • Do not add anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Include representative slides (will be returned)

Bone marrow

  • Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
    • Specimens collected in EDTA are acceptable, but not preferred
  • Transfer to an anticoagulant free sterile tube
  • If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable
  • Submit representative slides (will be returned)

Cerebrospinal fluid (CSF)

  • Collect 5 mL (minimum 1 mL) in a sterile vial/container.
    • Do NOT add any anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Send representative slides (will be returned)

Tissue

  • Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
  • Send representative slides (will be returned)
  

Body fluid

Falcon vial/tube - 15mL

Bone marrow

Bone marrow transport vial/tube

Cerebrospinal fluid (CSF)

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

  

Manual request clients: Complete the "Brief clinical information" and "What do you want answered by Flow?" areas on the request form. Include previous Allina case number, if applicable.

  

EDTA whole blood

Ambient

Body fluid

Refrigerated

Bone marrow

Ambient

Spinal fluid

Refrigerated

Tissue

Refrigerated

  
14 days
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
    • Tissues submitted in saline or B5 fixative are not acceptable and will be rejected
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Inappropriate timing of collection
  • Interfering substances
  • Leaking container
  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow cytometry

  

Tubes and Markers:

Tubes: A3 (8C), A4 (8C) and A5 (8C)

Markers: CD11b, CD13, CD14, CD15, CD33, CD34, CD38, CD45, CD56, CD64, CD65, CD117, CD123, CD371and HLA-DR

  
88184
88185 x 14
  
08/04/2021
  
08/05/2021