DAT negative evaluation
DAT negative evaluation-6994
DAT negative hemolytic anemia evaluation
Super coombs
- Monospecific DAT -IgG
- Monospecific DAT - C3
- Monospecific DAT -IgM
- Monospecific DAT - IgA
- Cold LISS wash solution using normal monospecific IgG
- Direct Polybrene using washed cells and one with unwashed cells and normal monospecific IgG
To help with the investigation of autoimmune hemolytic anemia when the DAT is negative.
Before ordering, consult with Blood Bank Pathologist.
Ideally the patient should not have been transfused in the last 3 months, but if they have been, a limitation note will be added to the results
Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting
Labelling instructions:
Excellian sites:
Tube must be labeled with the patients first and last name and Excellian MRN, date and time of collection and phlebotomist's A#
Non-Excellian sites:
Tube must be labeled with the patient's first and last name and date of birth, date and time of collection and phlebotomist's initials.
Lavender (EDTA), 4mL x 2
- Diagnosis
- Transfusion history in the last 3 months
- If yes, the # of units, type of units (ie RBC, FFP, Plt), and date of transfusion
- Transfusion history >3months
- If yes, dates
- Previous pregnancy
- If yes, number of pregnancies and dates
- Medication list
- IVIG given?
- If yes, dates
Refrigerated - 72 hours
Ambient - NO
Frozen - NO
- Improper label (unlabeled or mislabeled)
- Hemolysis
Serological
DAT is assessed using standard tube technique with anti-IgG, -C3, -IgM, and -IgA reagents. Smail amounts of bound immunoglobulin can be detected using Polybrene. Cold LISS wash technique is used to detect low affinity IgG.