RUNX/RUNX1 by FISH
RUNX/RUNX1 by FISH-4280G (bone marrow) or 4280H (blood)
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
Acute Myeloid Leukemia
FISH
Fluorescence in situ hybridization
t(8;21)
RUNX/RUNX1 or t(8;21) is a recurring abnormality in AML. According to current NCCN AML guidelines, a t(8;21) result is classified as a favorable cytogenetics risk factor.
This test can be ordered individually or as part of the AML FISH Panel. See the panel test for more details
Bone marrow aspirate:
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
- Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
- Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting
Transfer aspirate from syringe to transport container.
Submit entire specimen unspun.
Sterile vial/tube
or
Dk green Sodium heparin (Na hep), no gel
Sodium heparin (Na hep) whole blood
Touch prep slides
Bone marrow core:
Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline
Whole blood:
Dk green Sodium heparin (Na hep), no gel
Whole blood: 5 mL
Whole blood:
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting.
Submit entire specimen unspun
AHL Cytogenetics Oncology Request
Ambient
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Delay in transport
- Improper storage
- Leaking container
- Slides broken beyond repair
Fluorescence in situ hybridization (FISH)
Normal or Abnormal with interpretation