Dk green Sodium heparin (Na hep), no gel

Immediately following collection, mix sample by inverting 5 - 10 times to prevent clotting.

1. Spin
2. Transfer plasma to a Screw-cap polypropylene frozen transport vial/tube - 4 mL (LabCorp), labelled as Na hep plasma, within two (2) hours of sample collection
3. Freeze

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

Dk green Lithium heparin (Li hep), no gel

Lavender (EDTA), 4mL

Gold serum separator (SST) tube

Red serum vial/tube, 5 mL

Gold:

Immediatley following collection, mix sample by gently inverting 5 times

Lavender:

Immediatley following collection, mix sample by inverting 8 - 10 times to prevent clotting.

Dk green Li hep:

Immediately following collection, mix sample by inverting 5 - 10 times to prevent clotting.

Gold:

1. Allow sample to clot for a minimum of 30 minutes
2. Spin
3. Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum, within two (2) hours of sample collection

Red:

1. Allow sample to clot
2. Spin
3. Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum, within two (2) hours of sample collection

Lavender/Dk green:

1. Spin
2. Transfer plasma to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as EDTA or Li hep plasma, within two (2) hours of sample collection

Frozen (strict) - 14 days

• Gross hemolysis
• Ambient/room temperature or thawed specimens
• Specimens collected in yellow-top (ACD) tubes

Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

Note: Plasma reference ranges will be applied to all specimen types

Taurine:                    29.2 - 132.3 µmol/L
Aspartate:                   0.0 - 7.4 µmol/L
Hydroxyproline:           4.7 - 35.2 µmol/L
Threonine:                 67.8 - 211.6 µmol/L
Serine:                      48.7 - 145.2 µmol/L
Asparagine:               29.5 - 84.5 µmol/L
Glutamate:                18.1 - 155.9 µmol/L
Glutamine:               372.8 - 701.4 µmol/L
Sarcosine:                    0.0 - 4.0 µmol/L
Alpha-aminoadipate:     0.0 - 1.9 µmol/L
Proline:                      84.8 - 352.5 µmol/L
Glycine:                    144.0 - 411.0 µmol/L
Alanine:                    209.2 - 515.5 µmol/L
Citrulline:                    15.6 - 46.9 µmol/L
Alpha-aminobutyrate:    5.4 - 34.5 µmol/L
Valine:                      133.0 - 317.1 µmol/L
Cystine:                      15.8 - 47.3 µmol/L
Methionine:                 14.7 - 35.2 µmol/L
Homocitrulline:              0.0 - 1.7 µmol/L
Cystathionine:               0.0 - 0.7 µmol/L
Alloisoleucine:               0.0 - 3.2 µmol/L
Isoleucine:                  32.8 - 88.3 µmol/L
Leucine:                      66.7 - 165.7 µmol/L
Tyrosine:                     27.8 - 83.3 µmol/L
Phenylalanine:             35.8 - 76.9 µmol/L
Argininosuccinate:          0.0 - 3.0 µmol/L
Beta-alanine:                 1.1 - 9.0 µmol/L
Beta-aminoisobutyrate:  0.0 - 4.3 µmol/L
Homocysteine:               0.0 - 0.2 µmol/L