MiTF by IHC

Alphabetical Test listing

MiTF by IHC-12376 - Technical only, 12379 - Technical & Interpretation

Test name:

MiTF by IHC

Test number:

12376 - Technical only, 12379 - Technical & Interpretation

Excellian order number:

LAB12376
LAB12379

Alternate names:

Microphthalmia transcription factor

Tests included:

All IHC stains will include a positive control tissue

Useful for:

Anti MITF recognizes a nuclear protein which is expressed in most primary and metastatic epithelioid malignant melanomas
MITF is also seen in normal melanocytes, benign nevi and dysplastic nevi
This marker is present in a small subset of non-melanoma tissues and tumors (heart, macrophages, rare cells of pituitary, endometrial stromal cells, breast cancer, leiomyosarcoma, malignant mixed mullerian tumors, and renal cell carcinomas

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin-embedded (FFPE) tissue block

Transport container:

FFPE tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

MITF is a transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium
It is also involved in the to enzyme genes such as tyrosinase, TRP1 and TRP2

Staining pattern

Nuclear

References

Chang KL et al: Diagnostic utility of microphthalmia transcription factor in malignant melanoma and other tumors. Adv Anat Pathol. 2001; 8(5):273.
Granter SR et al: Rogle for MITF in the diagnosis of metastatic malignant melanoma. Appl Immunhistochem Mol Morphol 2002; 10(1):47.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

07/17/2017

Revised date:

10/19/2018

Review date:

10/02/2020