NKI/C3-MEL by IHC

Alphabetical Test listing

NKI/C3-MEL by IHC-12376 - Technical only, 12379 - Technical & Interpretation

  
NKI/C3-MEL by IHC
  
12376 - Technical only, 12379 - Technical & Interpretation
  
LAB12376
LAB12379
  
Melanoma-associated antigen
  
  • All IHC stains will include a positive control tissue
  
  • NkI/C-3 reacts with melanoma, nevocellular nevi, carcinoids, and medullary thyroid carcinoma
  • NkI/C-3 reacts with some normal tissues, including mast cells, histiocytes, salivary glands, bronchial glands, pancreatic and prostatic epithelium
  • NkI/C-3 has been identified in other tumors including prostate, breast, ovarian and lung carcinomas
  • Staining in non-melanoma tumors is usually only focal or atypical in contrast to diffuse or peripheral staining of melanocytic lesions
  • Due to its lack of specificity, NkI/C-3 should only be used in a panel with other melanoma markers (S100 and HMB45) and epithelial markers (CK or EMA)
  • One report showed strong positive staining with NkI/C-3 in atypical fibroxanthomas and DFSPs1. It should be noted however that all of these tumors were negative for S100 and HMB45
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • Melanoma-associated antigen that recognizes a glycoprotein located primarily in the inner side of cytoplasmic vesicles in melanoma cells

Staining pattern

  • Cytoplasmic based staining
  • Sometimes peripherally near the cell membrane

References

  1. Chan et. al. Immunohistochemical Characterization of Atypical Fibroxanthoma and Dermatofibrosarcoma Protuberans; American Journal of Clinical Pathology; 97:478-483, 1992
  
88342 - 1st stain
88341 - each additional stain
  
07/18/2017
  
06/25/2019