Alphabetical Test listing

NKI/C3-MEL by IHC-12376 - Technical only, 12379 - Technical & Interpretation

12376 - Technical only, 12379 - Technical & Interpretation
Melanoma-associated antigen
  • All IHC stains will include a positive control tissue
  • NkI/C-3 reacts with melanoma, nevocellular nevi, carcinoids, and medullary thyroid carcinoma
  • NkI/C-3 reacts with some normal tissues, including mast cells, histiocytes, salivary glands, bronchial glands, pancreatic and prostatic epithelium
  • NkI/C-3 has been identified in other tumors including prostate, breast, ovarian and lung carcinomas
  • Staining in non-melanoma tumors is usually only focal or atypical in contrast to diffuse or peripheral staining of melanocytic lesions
  • Due to its lack of specificity, NkI/C-3 should only be used in a panel with other melanoma markers (S100 and HMB45) and epithelial markers (CK or EMA)
  • One report showed strong positive staining with NkI/C-3 in atypical fibroxanthomas and DFSPs1. It should be noted however that all of these tumors were negative for S100 and HMB45

Submit a formalin-fixed, paraffin embedded tissue block


Formalin-fixed, paraffin embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • Melanoma-associated antigen that recognizes a glycoprotein located primarily in the inner side of cytoplasmic vesicles in melanoma cells

Staining pattern

  • Cytoplasmic based staining
  • Sometimes peripherally near the cell membrane


  1. Chan et. al. Immunohistochemical Characterization of Atypical Fibroxanthoma and Dermatofibrosarcoma Protuberans; American Journal of Clinical Pathology; 97:478-483, 1992
88342 - 1st stain
88341 - each additional stain