Beta amyloid by IHC

Alphabetical Test listing

Beta amyloid by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
Beta amyloid by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
IHC
  
  • All IHC stains will include a positive control tissue
  
  • Beta amyloid is used to identify amyloid angiopathy, and is more sensitive than congo red stains
  • This antibody can also identify Alzheimer's neurofibrillary tangles
  • This antibody will not identify the other types of amyloid (use kappa and lambda to identify primary amyloidosis/plasma cell dyscrasias; we don't have antibodies to the SAA (serum amyloid associated protein) or beta 2 microglobulin protein types of amyloid
  
Tissue
  

Prepare a formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue block

  

Tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • Beta amyloid is an extracellular filamentous protein deposit found in the brain, and is the major protein of amyloid cores, neuritic plaques, and neurofibrillary tangles
  • This protein is found in Alzheimer's disease, and other rare types of dementia
  • Beta amyloid is also found in amyloid angiopathy, which is a leading cause of spontaneous intracerebral hemorrhage in the elderly

Staining patterns

  • Cytoplasmic staining

References

  1. Ishihara T et al: The significance of cerebrovascular amyloid in the aetiology of superficial (lobar) cerebral hemorrhage and its incidence in the elderly population. J Pathol 1991; 165:229-234.
  2. Vinters HV et al: Immunohistochemical study of cerebral amyloid angiopathy. III: Widespread Alzheimer A4 peptide in cerebral micro-vessel walls co-localizes with gamma trace in patients with leukoencephalopathy. Ann Neurol 1990; 28: 34-42.
  
88342 - 1st stain
88341 - each additional stain
  
05/11/2017
  
10/17/2018
  
10/02/2020