KIT Asp816Val mutation analysis

Alphabetical Test listing

KIT Asp816Val mutation analysis-994

Test name:

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Alternate names:

C-KIT
D816V
Systemic mastocytosis mutation
tumors of hematopoietic tissue

Useful for:

Diagnosing systemic mastocytosis using blood or bone marrow specimens

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL

Volume:

3.0 mL

Minimum volume:

1.0 mL

Collection instructions:

Immediatley following collection, mix sample by inverting 8 – 10 times to prevent clotting

Transport container:

Lavender (EDTA), 4mL

Alternate specimen type:

Bone marrow
Extracted DNA from blood or bone marrow

Alternate collection container:

Yellow ACD (A or B)

Alternate specimen volume:

Bone marrow – 2 mL
Extracted DNA - 50 µL at 20 ng/µL concentration

Alternate transport container:

Bone marrow:

Lavender (EDTA), 4mL

Yellow ACD (A or B)

Extracted DNA:

1.5 to 2mL screw-top tube (extracted DNA)

Required forms/information:

Provide indication of volume and concentration of DNA extracted

Molecular Medicare billing request

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.

Complete and submit the form to notify us of the need for Allina Health Laboratory to bill insurance for Molecular testing performed
All information requested is required in order for your request to be completed

Transport and stability:

Peripheral blood or bone marrow:

Ambient (preferred) – 7 days

Refrigerated – 7 days

Frozen – NO

Extracted DNA from blood or bone marrow:

Ambient (preferred) – 7 days

Refrigerated – 7 days

Frozen (OK)

Specimen retention time:

Peripheral blood, bone marrow: 2 weeks
DNA: 3 months

Reason for rejection:

Gross hemolysis
Moderately to severely clotted
Bone marrow biopsies
Paraffin-embedded bone marrow clots
Paraffin-embedded tissue
Slides
Paraffin shavings

Performing lab:

Mayo Clinic Laboratories (KITVS): R-NX

Days set up:

Mo - Fr

TAT:

7 days

Method:

Allele-Specific Oligonucleotide Polymerase Chain Reaction (PCR)

Reference ranges:

An interpretive report will be provided indicating the mutation status as positive or negative.

Preauthorization:

This test may require preauthorization from the insurance provider. Check the payer guidelines and, if needed, obtain the pre-authorization prior to sample collection.

CPT codes:

81273

Subject to medical necessity (Y/N):

Yes

LCD/NCD:

Medical necessity

Date created:

09/23/2021

Revised date:

03/21/2024

Review date:

01/02/2024