MLH1 promoter methylation analysis, tumor

Alphabetical Test listing

MLH1 promoter methylation analysis, tumor-Pathology add-on

  
MLH1 promoter methylation analysis, tumor
  
Pathology add-on
  
Hypermethylation
MLH1HM
MLH1 hypermethylation
Promoter hypermethylation
  

MLH1 promoter methylation analysis is useful to distinguish sporadic from inherited colorectal and endometrial cancers in tumors that are MLH1-deficient by IHC staining and/or have high levels of microsatellite instability (MSI-H). The majority of MSI in sporadic cases of these tumors is caused by MLH1 promoter hypermethylation, while hypermethylation is rare in inherited cases.

  
Solid tumor tissue
  

Submit a formalin-fixed, paraffin-embedded (FFPE) tissue block (preferred)

  

FFPE tissue block

  
  • One (1) H&E slide plus five to 10 (5-10) unstained slides cut at 5 or more microns.
    • Use positively-charged slides and 10% NBF fixative
    • Do not use zinc fixatives
  

Ambient

 

  
Neogenomics: R
  
10 days
  

Polymerase Chain Reaction (PCR) Analysis

  

An interpretive report will be provided.

  

MLH1 promoter methylation analysis results should be considered with other clinical risk factors in determination of likelihood of HNPCC/Lynch Syndrome.

  
81288
  
Yes
  
  
17028037
  
308128800
  
06/15/2018
  
05/12/2020
  
05/12/2020