BCL6 by FISH

Alphabetical Test listing

BCL6 by FISH-4280J

  
BCL6 by FISH
  
4280J
  
LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES
  
CG BCL6 or BCL6
  
BCL6
Diffuse large B-cell lymphoma
FISH
Fluorescence in situ hybridization
High grade B-cell lymphoma
Large B-cell lymphoma
3q27
  

To identify patients with BCL6 rearrangements associated with large b-cell lymphoma to help differentiate diffuse large b-cell lymphoma from high grade lymphoma.

This test can be ordered individually or as part of a High Grade Lymphoma FISH Panel (See the panel test for details).

  
Involved tissue (solid tumor or other)
  

Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline.

  
5 mm (3)
  

Specimens should be immersed in sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one (1) hour of the procedure

  

Sterile culture medium or in a sterile cup with sterile saline

  
Tumor tissue FFPE block
Tumor tissue FFPE slides
  
Block
Slides: 2 plus one H & E stained slide
  
  1. Fix the tissue in 10% neutral buffered formalin for 6 - 72 hours
  2. Prepare a formalin-fixed, paraffin-embedded (FFPE) tissue block

Either the entire block (an H&E will be cut and stained, and assessed by an HPA pathologist), or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing

  

FFPE tissue block or

Tissue section mounted on a charged, unstained slides

  
  • AHL Cytogenetics oncology request
  

Ambient

  
Paraffin specimens:
Slides - 6 months
Blocks will be returned post reporting

Fresh tissue – until testing is reported
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Delay in transport
  • Improper storage
  • Leaking container
  • Slides broken beyond repair
  
AHL - Cytogenetics: X
  
2 - 3 times per week
  
5 days
  

Fluorescence in situ hybridization (FISH)

  

Normal or Abnormal with interpretation

  
Paraffin embedded specimens:
88366x1 – Qualitative Morphometric FISH.

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred

Fresh tissue specimens:
88271x2
88275x1
  
Yes
  
  
03/26/2021