Flow cytometry (non-peripheral blood)

Alphabetical Test listing

Flow cytometry (non-peripheral blood)-12379

  
Flow cytometry (non-peripheral blood)
  
12379
  
LAB12420
  
PATR
  
B- lymphocyte
Leukemia/Lymphoma panel
Myeloma
Lymphocyte subset
T-lymphocyte
  
Body fluid
  
20 mL
  
5 mL
  
  • Place body fluid in a Falcon vial/tube - 15mL
    • Do not add anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Include representative slides (will be returned)
  
  
Bone marrow
Spinal fluid
Tissue
  
Varies; see Alternate collection instructions
  

Bone marrow

  • Collect 3 mL (minimum 1.0 mL) in a sodium heparinized (Na hep) syringe
    • Specimens collected in EDTA are acceptable, but not preferred
  • Transfer to an anticoagulant free sterile tube
  • FI unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable
  • Submit representative slides (will be returned)

Spinal fluid

  • Collect 5 mL (minimum 1.0 mL) in a sterile vial/container.
    • Do NOT add any anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Send representative slides (will be returned)

Tissue

  • Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
  • Send representative slides (will be returned)
  

Bone marrow

Bone marrow transport vial/tube

 

Spinal fluid

Sterile vial/container

 

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

  

Complete the "Brief clinical information" and "What do you want answered by Flow?" areas on the request form

  

Body fluid

Refrigerated

Bone marrow

Ambient

Spinal fluid

Refrigerated

Tissue

Refrigerated

  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Inappropriate timing of collection
  • Interfering substances
  • Leaking container
  • Slides broken beyond repair
  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow Cytometry

  

Flow cytometry may be requested as part of any surgical or cytology case by manually indicating “Please perform flow cytometry” in the comment field of the AP order

Flow cytometry panel CD's

  
Varies by panel
  
02/24/2003
  
08/28/2018
  
02/24/2020