Cytokeratin 5/6 by IHC

Alphabetical Test listing

Cytokeratin 5/6 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Cytokeratin 5/6 by IHC
12376 - Technical only, 12379 - Technical & interpretation
CK 5/6

All IHC stains will include a positive control tissue

  • This antibody is useful in identifying hyperplastic breast epithelium (CK5/6 positive)
  • Neoplastic breast proliferations (ADH, DCIS) are typically CK5/6 negative
  • CK5/6 also is a marker of breast myoepithelial cells
  • This antibody will also stain squamous epithelium of normal skin
  • CK5/6 is useful in separating mesotheliomas from pulmonary adenocarcinomas; it will stain mesothelial cells and mesotheliomas, but it is typically negative in adenocarcinomas

Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • CK5/6 is an antibody cocktail that reacts with high-molecular weight keratins (56,58 kD)

Staining pattern

  • Cytoplasmic


  1. Cury PM et al; Mod Pathol 2000;13:107-12.
  2. Otterback F et al: Histopathology 2000;37:232-40.
88342 - 1st stain
88341 - each additional stain