SS18-SSX by IHC

Alphabetical Test listing

SS18-SSX by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
SS18-SSX by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  

All IHC stains will include a positive control tissue

  

Monoclonal antibody targeting the SS18-SSX fusion protein. 

  • Reported sensitivity of 87-95% with 100% specificity when strong diffuse nuclear positivity is present (expression in greater than 75% of tumor cells).
  • Decreased sensitivity has been reported in decalcified specimens, specimens with delayed tissue fixation, areas of necrosis and in areas of crush artifact.
  
Tissue
  

Prepare a formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue block

  

Tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

Staining pattern:  Nuclear

 

The below table from Zaborowski et al highlights an algorithm for the use and interpretation of SS18-SSX.

 

References:

  1. Perret et al. The SS18-SSX antibody has perfect specificity for the SS18-SSX Fusion protein.  Am J Surg Pathol 2021;45(5):582-583.
  2. Baranov et al. A novel SS18-SSX fusion-specific antibody for the diagnosis of synovial sarcoma. Am J Surg Pathol 2020;44:922-933.
  3. Zaborowski et al. When used together SS18-SSX fusion-specific and SSX C-terminus immunohistochemistry are highly specific and sensitive for the diagnosis of synovial sarcoma and can replace FISH or molecular testing in most cases. Histopathology 2020;77:588-600.
  
88342 - 1st stain
88341 - each additional stain
  
04/29/2021
  
04/30/2021