SS18-SSX by IHC

Alphabetical Test listing

Test name:

SS18-SSX by IHC

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Tests included:

All IHC stains will include a positive control tissue

Useful for:

Monoclonal antibody targeting the SS18-SSX fusion protein.

Reported sensitivity of 87-95% with 100% specificity when strong diffuse nuclear positivity is present (expression in greater than 75% of tumor cells).
Decreased sensitivity has been reported in decalcified specimens, specimens with delayed tissue fixation, areas of necrosis and in areas of crush artifact.

Specimen type:

Tissue

Processing instructions:

Prepare a formalin-fixed, paraffin-embedded (FFPE) tissue block

Transport container:

FFPE tissue block

Alternate transport container:

Tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Clinical information:

Staining pattern: Nuclear

The below table from Zaborowski et al highlights an algorithm for the use and interpretation of SS18-SSX.

References:

Perret et al. The SS18-SSX antibody has perfect specificity for the SS18-SSX Fusion protein. Am J Surg Pathol 2021;45(5):582-583.
Baranov et al. A novel SS18-SSX fusion-specific antibody for the diagnosis of synovial sarcoma. Am J Surg Pathol 2020;44:922-933.
Zaborowski et al. When used together SS18-SSX fusion-specific and SSX C-terminus immunohistochemistry are highly specific and sensitive for the diagnosis of synovial sarcoma and can replace FISH or molecular testing in most cases. Histopathology 2020;77:588-600.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

04/29/2021

Review date:

01/12/2024