The detection of anti-skin (IC and BMZ) antibodies aids in the diagnosis, and sometimes prognosis, of chronic vesicular-bullous diseases including pemphigus, pemphigoid, cicatricial pemphigoid, and epidermolysis bullosa acquisita (EBA).
Spin, separate and freeze
Frozen (preferred) - 1 year
Refrigerated - 5 days
Ambient - 48 hours
Semi-quantitative Indirect Immunofluorescence (IFA)
Substrate: Rhesus Monkey and Guinea Pig Esophagus/NaCl Split-Skin Substrate
An interpretive report will be provided
Epithelial intercellular antibodies are diagnostic for pemphigus and occur in over 90% of patients with active forms. Because of the species specificity of intercellular antibodies, the use of the dual substrates further distinguishes the intercellular antibodies of pemphigus vulgaris and vegetans from the antibodies of pemphigus foliaceus and erythematosus. Antibodies to basement membrane antigens of stratified squamous epithelium occur in about 70% of active bullous pemphigoid, 50% of vesicular pemphigoid and EBA and 10% of cicatricial pemphigoid patients.