CD35 by IHC

Alphabetical Test listing

CD35 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
CD35 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Anti-human dendritic reticulum cell
  

All IHC stains will include a positive control tissue

  
  • CD35 is useful in identifying the follicular dendritic cells in non-Hodgkin's lymphomas, seen as a sharply defined area of nodular staining
  • This antibody can also show a loose, ill-defined area of staining in some diffuse lymphomas
  • Precursor B NHL such as lymphoblastic lymphomas, and Burkitt lymphomas, plasmocytomas, and hairy cell leukemias show no staining for CD35
  • Nodular lymphocyte predominant Hodgkin's disease will show CD35 staining in a follicular pattern; all other types of HD are negative
  • CD 35 is most useful in a panel with CD21 and CD23 to identify follicular dendritic cell sarcoma/tumor
  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • CD35 antibody reacts with a receptor of the C3b fragment of compliment (To5)
  • CD35 labels follicular dendritic cells in lymphoid follicles, and to a lesser degree, mantle zone lymphocytes
  • CD35 stains the C3b receptor on epithelial cells of renal glomeruli
  • CD35 will also stain macrophages and histiocytes

Staining pattern

  • Membrane -based staining

 

References

  1. Chan JKC: Proliferative lesions of follicular dendritic cells: an overview, including a detailed account of follicular dendritic cell sarcoma, a neoplasm with many faces and uncommon etiologic associations. Adv Anat Pathol, 1997; 4(6); 347-411.
  2. Nguyen DT et al: Follicular dendritic cell sacrum. Identification by monoclonal antibodies in paraffin sections. Appl Immunohistochem 2:60-64.
  
88342 - 1st stain
88341 - each additional stain
  
06/16/2017
  
10/17/2018
  
10/02/2020