Cytokeratin 7 by IHC

Alphabetical Test listing

Cytokeratin 7 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
Cytokeratin 7 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
CK7
  

All IHC stains will include a positive control tissue

  
  • CK7 is found in a large number of cell types including many ductal and glandular epithelia (biliary and pancreatic ducts, lung alveoli, breast, ovary, endometrium, renal collecting ducts, urothelium, thyroid, placental trophoblasts, and mesothelium)
  • CK7 and CK20 can be helpful in determining the site of origin of tumors, based on the staining profile
Immunophenotype Tumor
CK20+/CK7+ Ovarian mucinous carcinoma
Pancreatic carcinoma
Transitional cell carcinoma
CK20-/CK7+ Breast carcinoma, both ductal and lobular types
Bronchoalveolar carcinoma of lung
Endometrial carcinoma
Malignant mesothelioma
Non-small cell adenocarcinoma of lung
Ovarian carcinoma other than mucinous tumor
CK20+/CK7- Colorectal adenocarcinoma*
CK20-/CK7- Hepatocellular carcinoma
Prostatic adenocarcinoma
Renal cell carcinoma
Small cell neuroendocrine carcinoma of lung
Squamous cell carcinoma of lung

*Reduced or absent CK20 activity can be seen in colorectal carcinomas with high levels of microsatellite instability.

Tumor subtype CK7/CK20 profile Fraction of cases positive (%)
Bladder transitional cell
carcinoma
CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
17/19 (89%)
2/19 (11%)
0/19 ( 0%)
0/19 (0%)
Colorectal adenocarcinoma CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
4/40 (10%)
0/40 (0%)
30/40 (75%)
6/40 (15%)
Gastric adenocarcinoma CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
11/29 (38%)
5/29 (17%)
10/29 (35%)
3/29 (10%)
Pancreatic adenocarcinoma CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
15/23 (65%)
6/23 (26%)
2/23 (9%)
0/23 (0%)
Lung, non-small cell
carcinoma
CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
6/54 (11%)
39/54 (72%)
0/54 (0%)
9/54 (17%)
Lung, bronchiolo-alveolar
carcinoma
CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
0/3 (0%)
3/3 (100%)
0/3 (0%)
0/3 (0%)
Lung, squamous cell
carcinoma
CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
0/12 (0%)
0/12 (0%)
1/12 (8%)
11/12 (92%)
Lung, small cell carcinoma CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
0/11 (0%)
2/11 (18%)
0/11 (0%)
9/11 (82%)
Hepatocellular carcinoma CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
2/30 (7%)
5/30 (17%)
0/30 (0%)
23/30 (77%)
Ovary, serous and
endometrioid carcinoma
CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
0/19 (0%)
19/19 (100%)
0/19 (0%)
0/19 (0%)
Endometrial carcinoma CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
3/25 (12%)
20/25 (80%)
0/25 (0%)
2/25 (8%)
Prostatic adenocarcinoma CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
1/13 (8%)
1/13 (8%)
3/13 (23%)
8/13 (62%)
Renal cell carcinoma CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
0/17 (0%)
4/17 (24%)
1/17 (6%)
12/17 (71%)
Breast, infiltrating ductal
carcinoma
CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
6/38 (16%)
31/38 (82%)
1/38 (3%)
0/38 (0%)
Breast, infiltrating lobular
carcinoma
CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
1/11 (9%)
10/11 (91%)
0/11 (0%)
0/11 (0%)
Malignant mesothelioma CK7+/CK20+
CK7+/CK20-
CK7-/CK20+
CK7-/CK20-
0/16 (0%)
11/16 (69%)
0/16 (0%)
5/16 (31%)

 

  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

 Specifications

  • CK7 antibody recognizes human cytokeratin 7 intermediate filament protein, found in glandular and transitional epithelium

 
Staining pattern

  • Cytoplasmic based

References

  1. Berezowski K, Stastny JF, Kornstein MJ: Cytokeratins 7 and 20 and carcinoembryonic antigen in ovarian and colonic carcinoma. Mod Pathol 1996;9(4):426-429.
  2. Campbell F, Herrington CS: Application of cytokeratin 7 and 20 immunohistochemistry to diagnostic pathology. Curr Diagn Pathol 2001;7:113-122.
  3. Chu P, Wu E, Weiss LM: Cytokeratin 7 and cytokeratin 20 expression in epithelial neoplasms: a survey of 435 cases. Mod Pathol 2000;13(9):962-972.
  4. Wang et.al., Appl Immunohistochem 3:99-107, 1995.
  
88342 - 1st stain
88341 - each additional stain
  
12/19/2017
  
10/18/2018
  
10/02/2020